Karin J. von Eije

TL;DR: Whereas HIV-1 could escape from a single shRNA, it is shown that HIV- 1 escape can be prevented when four shRNAs are simultaneously expressed in a cell.

TL;DR: Insight is obtained in the viral escape routes when essential and highly conserved sequences are targeted in the Gag, protease, integrase, and Tat-Rev regions of HIV-1 and the type of mismatch in the siRNA-target RNA duplex provides insight into the sequence requirements for optimal RNAi inhibition.

TL;DR: E-shRNAs can be used as a combinatorial RNAi approach to target error-prone viruses and it is shown that HIV-1 replication is durably inhibited in T cells stably transduced with a lentiviral vector containing the e3-shRNA expression cassette.

TL;DR: In this paper , the authors investigated the global SARS-CoV-2 genomic surveillance, using sequences shared via GISAID to estimate the impact of sequencing intensity and turnaround times on variant detection in 189 countries.

TL;DR: Five cases of imported Zika virus infection to The Netherlands are reported, establishing the diagnosis is important, mainly because of the association with congenital microcephaly and the possibility of sexual transmission.