N
Nick C.T. Schopman
Researcher at University of Amsterdam
Publications - 10
Citations - 595
Nick C.T. Schopman is an academic researcher from University of Amsterdam. The author has contributed to research in topics: RNA interference & Small hairpin RNA. The author has an hindex of 9, co-authored 10 publications receiving 558 citations.
Papers
More filters
Journal ArticleDOI
Deep sequencing of virus-infected cells reveals HIV-encoded small RNAs
Nick C.T. Schopman,Marcel Willemsen,Ying Poi Liu,Ted E.J. Bradley,Antoine H. C. van Kampen,Frank Baas,Ben Berkhout,Joost Haasnoot +7 more
TL;DR: It is suggested that HIV-1 triggers the production of vsiRNAs and vmiRNAs to modulate cellular and/or viral gene expression and suppression of the vsi RNAs by antagomirs stimulate virus production.
Journal ArticleDOI
A miRNA-tRNA mix-up: tRNA origin of proposed miRNA.
TL;DR: The results suggest that next generation sequencing approaches are particularly prone to such misannotations, and it is proposed that the corresponding miRNAs should be validated in more detail, as the small RNA fragments that led to their description are likely derived from tRNA processing.
Journal ArticleDOI
Combinatorial RNAi Against HIV-1 Using Extended Short Hairpin RNAs
Ying Poi Liu,Karin J. von Eije,Nick C.T. Schopman,Jan-Tinus Westerink,Olivier ter Brake,Joost Haasnoot,Ben Berkhout +6 more
TL;DR: E-shRNAs can be used as a combinatorial RNAi approach to target error-prone viruses and it is shown that HIV-1 replication is durably inhibited in T cells stably transduced with a lentiviral vector containing the e3-shRNA expression cassette.
Journal ArticleDOI
Dicer-independent processing of short hairpin RNAs
TL;DR: Detailed analyses indicated that a short shRNA stem length is critical for avoiding Dicer processing and activation of the alternative processing route, in which the shRNA is incorporated into RISC and processed by the AGO2-mediated slicer activity.
Journal ArticleDOI
Anticipating and blocking HIV-1 escape by second generation antiviral shRNAs
TL;DR: This work presents a strategy that anticipates HIV-1 escape by designing 2nd generation short hairpin RNAs (shRNAs) that form a complete match with the viral escape sequences and demonstrates the power of the 2 second generation RNAi concept.