Showing papers by "Karine G. Le Roch published in 2018"
University of Texas Health Science Center at San Antonio1, University of California, Riverside2, University of Washington3, Johns Hopkins University4, Mahidol University5, University of Oxford6, Columbia University Medical Center7, University of Nottingham8, Columbia University9, La Jolla Institute for Allergy and Immunology10
TL;DR: The authors show that the three-dimensional genome structure of human malaria parasites is strongly connected with transcriptional activity of specific gene families throughout the life cycles of Plasmodium falciparum and Plas modium vivax parasites.
Abstract: The development of malaria parasites throughout their various life cycle stages is coordinated by changes in gene expression. We previously showed that the three-dimensional organization of the Plasmodium falciparum genome is strongly associated with gene expression during its replication cycle inside red blood cells. Here, we analyze genome organization in the P. falciparum and P. vivax transmission stages. Major changes occur in the localization and interactions of genes involved in pathogenesis and immune evasion, host cell invasion, sexual differentiation, and master regulation of gene expression. Furthermore, we observe reorganization of subtelomeric heterochromatin around genes involved in host cell remodeling. Depletion of heterochromatin protein 1 (PfHP1) resulted in loss of interactions between virulence genes, confirming that PfHP1 is essential for maintenance of the repressive center. Our results suggest that the three-dimensional genome structure of human malaria parasites is strongly connected with transcriptional activity of specific gene families throughout the life cycle.
79 citations
University of Texas Health Science Center at San Antonio1, University of Washington2, University of California, Berkeley3, University of California, Riverside4, Johns Hopkins University5, Mahidol University6, Columbia University Medical Center7, University of Nottingham8, Columbia University9, La Jolla Institute for Allergy and Immunology10, Mines ParisTech11
TL;DR: The results suggest that the three-dimensional genome structure is strongly connected with transcriptional activity of specific gene families throughout the life cycle of human malaria parasites.
Abstract: The development of malaria parasites throughout their various life cycle stages is controlled by coordinated changes in gene expression. We previously showed that the three-dimensional organization of the P. falciparum genome is strongly associated with gene expression during its replication cycle inside red blood cells. Here, we analyzed genome organization in the P. falciparum and P. vivax transmission stages. Major changes occurred in the localization and interactions of genes involved in pathogenesis and immune evasion, erythrocyte and liver cell invasion, sexual differentiation and master regulation of gene expression. In addition, we observed reorganization of subtelomeric heterochromatin around genes involved in host cell remodeling. Depletion of heterochromatin protein 1 (PfHP1) resulted in loss of interactions between virulence genes, confirming that PfHP1 is essential for maintenance of the repressive center. Overall, our results suggest that the three-dimensional genome structure is strongly connected with transcriptional activity of specific gene families throughout the life cycle of human malaria parasites.
25 citations
TL;DR: Understanding of the molecular mechanisms driving this process by characterizing an early factor in gametocytogenesis is extended, and how this fits neatly into current knowledge of sexual commitment is shown.
10 citations