Karl H. Rieckmann

TL;DR: Two enzymes involved in the de novo synthesis of pyrimidines, orotic acid PRTase, and orotidine 5'-phosphate decarboxylase, were present in parasite extracts as were the enzymes for pyrimidine nucleotide phosphorylation.

TL;DR: A new procedure for isolating spontaneously released merozoites from in vitro cultures of Plasmodium falciparum (FVO and FCB strains) is described and it is shown that purifiedmerozoite preparations will be invaluable for malaria immunization studies, for identification of protective antigens of P. falcIParum, and for other immunological and biochemical studies.

TL;DR: Studies were initiated with pyri- methamine and sulphadiazine in order to develop a system for monitoring the susceptibility of parasites to sulphonamide-pyrimethamine combinations, such as Fansidar, which are widely used in the treatment of chloroquine-resistant infections.

TL;DR: Further studies are indicated to determine to what extent findings obtained with the in vitro microtechnique can be correlated with the response of falciparum infection to treatment with pyrimethamine-sulphadoxine combinations.

TL;DR: The results indicate that this rabbit-in vitro model system may be a useful system for identifying potential agents against drug-resistant falciparum malaria, particularly compounds which are converted in vitro to their active metabolites or which exert a prolonged suppressive activity after drug administration.