K
Keiko Muguruma
Researcher at Kansai Medical University
Publications - 50
Citations - 7517
Keiko Muguruma is an academic researcher from Kansai Medical University. The author has contributed to research in topics: Induced pluripotent stem cell & Embryonic stem cell. The author has an hindex of 18, co-authored 47 publications receiving 6508 citations. Previous affiliations of Keiko Muguruma include Osaka Bioscience Institute & Setsunan University.
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Journal ArticleDOI
A ROCK inhibitor permits survival of dissociated human embryonic stem cells
Kiichi Watanabe,Morio Ueno,Daisuke Kamiya,Ayaka Nishiyama,Michiru Matsumura,Takafumi Wataya,Jun Takahashi,Satomi Nishikawa,Shin-Ichi Nishikawa,Keiko Muguruma,Yoshiki Sasai +10 more
TL;DR: Application of a selective Rho-associated kinase (ROCK) inhibitor, Y-27632, to hES cells markedly diminishes dissociation-induced apoptosis, increases cloning efficiency and facilitates subcloning after gene transfer, and enables SFEB-cultured hES Cells to survive and differentiate into Bf1+ cortical and basal telencephalic progenitors.
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Self-formation of optic cups and storable stratified neural retina from human ESCs.
Tokushige Nakano,Satoshi Ando,Nozomu Takata,Masako Kawada,Keiko Muguruma,Kiyotoshi Sekiguchi,Koichi Saito,Shigenobu Yonemura,Mototsugu Eiraku,Yoshiki Sasai +9 more
TL;DR: It is demonstrated that an optic cup structure can form by self-organization in human ESC culture and an optimized vitrification method enables en bloc cryopreservation of stratified neural retina of human origin.
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Self-Organized Formation of Polarized Cortical Tissues from ESCs and Its Active Manipulation by Extrinsic Signals
Mototsugu Eiraku,Kiichi Watanabe,Mami Matsuo-Takasaki,Masako Kawada,Shigenobu Yonemura,Michiru Matsumura,Takafumi Wataya,Ayaka Nishiyama,Keiko Muguruma,Yoshiki Sasai +9 more
TL;DR: Self-organized formation of apico-basally polarized cortical tissues from ESCs using an efficient three-dimensional aggregation culture (SFEBq culture) is demonstrated and spatial and temporal aspects of early corticogenesis are recapitulated and can be manipulated in this ESC culture.
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Self-Organization of Polarized Cerebellar Tissue in 3D Culture of Human Pluripotent Stem Cells
TL;DR: Human-ESC-derived cerebellar progenitors exhibit substantial self-organizing potential for generating a polarized structure reminiscent of the early human cerebellum at the first trimester.
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Self-formation of functional adenohypophysis in three-dimensional culture
Hidetaka Suga,Taisuke Kadoshima,Maki Minaguchi,Masatoshi Ohgushi,Mika Soen,Tokushige Nakano,Nozomu Takata,Takafumi Wataya,Keiko Muguruma,Hiroyuki Miyoshi,Shigenobu Yonemura,Yutaka Oiso,Yoshiki Sasai +12 more
TL;DR: Efficient self-formation of three-dimensional adenohypophysis tissues in an aggregate culture of mouse embryonic stem (ES) cells is reported, indicating functional anterior pituitary tissue self-forms in ES cell culture, recapitulating local tissue interactions.