Keith M. Derbyshire

TL;DR: The ease and efficiency of the procedure eliminate any requirement for genetic selection of mutants and thus allow phenotypically silent mutations to be obtained.

TL;DR: The insertion sequence IS903 has perfect, 18-base-pair terminal repeats that are the presumed binding sites of its transposase and it is inferred that the inverted repeat contains two functional domains--one for initial complex formation with transpos enzyme and the other for effective completion of transpositional recombination.

TL;DR: It is shown that there is a strong correlation between the stability of transposase and its ability to act in trans and that the La protease, product of the lon gene, is an important determinant ofTransposase instability.

TL;DR: Elegant biochemical studies have firmly established that Mu transposition is generally replicative and, in addition, have elucidated the structure of an intermediate formed through the action of the Mu transposase (A protein), the B protein, and the E. coli histone-like protein HU.

TL;DR: A derivative of the IS903 transposon (Tn) is described that is capable of creating lacZ gene fusions upon transposition and should find wide use as a tool for Tn mutagenesis in bacteria since it can be used both to generate mutants and to examine gene expression.