Kem A. Sochacki

TL;DR: These findings strongly suggest that disruption of the cytoplasmic membrane is not the growth-halting mechanism, and enable dissection of antimicrobial design criteria into two parts: translocation across the OM and the subsequent halting of growth.

TL;DR: It is proposed that endocytosis is driven by the recruitment, reorganization and loss of proteins within these partitioned nanoscale zones within which the presence or concentrations of proteins vary at distinct stages of organelle development.

TL;DR: Super-resolution localization fluorescence microscopy with transmission electron microscopy of metal replicas is used to locate proteins on the landscape of the cellular plasma membrane at the nanoscale and finds the previously unknown three-dimensional position of the endocytic protein epsin on clathrin-coated structures at the plasma membrane.

TL;DR: The results support the notion that BSC-1 mammalian cells dynamically regulate the flat-to-curved transition in clathrin-mediated endocytosis by both biochemical and mechanical factors.

TL;DR: The effective axial diffusion coefficient of green fluorescent protein (D(GFP) in the cytoplasm of E. coli cells as a function of (phi) for both plasmolyzed and adapted cells is measured and it is shown that the data cannot be explained by crowding theory.