Kirsten C. Weining

TL;DR: The type and the dose of cytokine genes injected into chickens influence the quality of the local immune response to DNA vaccination against coccidiosis, and Flow cytometric analysis of duodenum intraepithelial lymphocytes showed chickens that received the pcDNA3-1E vaccine simultaneously with the IL-8 or IL-15 genes had significantly increased CD3 + cells.

TL;DR: Sequence homology and structural features indicate that this protein is the chicken homolog of mammalian interleukin-1beta (ChIL-1 beta), and northern blot analysis showed that ChIL- 1 beta RNA is quickly induced in blood monocyte-derived macrophages reaching maximal levels within one hour after onset of LPS treatment.

TL;DR: Bacterially expressed ChIL-18 in which the N-terminal 29 amino acids of the putative precursor molecule were replaced by a histidine tag induced the synthesis of interferon-gamma in cultured primary chicken spleen cells, indicating that the recombinant protein is biologically active.

TL;DR: Supernatants of the chicken T cell line 855 contain antiviral and macrophage‐activating factor activity and strongly activate transcription of the guanylate‐binding protein (GBP) gene in chicken cells, and a cDNA expression cloning strategy in COS cells revealed that it encodes chicken interferon‐γ (ChIFN‐γ).

TL;DR: Two cDNA clones were isolated that code for polypeptides with structural features of chemokines that are similar to previously characterized chicken protein with homology to mammalian macrophage inflammatory protein 1beta and about 50% sequence identity to human MIP-1beta and other related CC chemokine.