Kiyoshi Tajima

TL;DR: The real-time PCR technique uncovered differential amplification of rumen bacterial templates with the set of universal bacterial primers, which may explain why some predominant rumen bacteria have not been detected in PCR-generated 16S ribosomal DNA libraries.

TL;DR: The bacterial community structure was revealed by phylogenetic placement of sequences in relation to different fractions of rumen content and the vast majority of sequences from the rumen solids were found to be related to low G+C Gram-positive bacteria and the remaining sequences were placed within the Cytophaga-Flexibacter-Bacteroides and Spirochaetes phyla.

TL;DR: Transitional changes of the ruminal bacterial community structure in cows during the switch from roughage to high-grain diet were monitored by PCR amplification and sequencing of 16S rDNA clone libraries.

TL;DR: Tannin sources containing both HT and CT were more potent in suppressing methanogenesis than those containing only HT, and tannins increased in vitro pH, reduced total VFA concentrations, increased propionate concentrations, and decreased concentrations of iso-acids.

TL;DR: Phylogenetic placement of eight almost complete 16S rRNA sequences revealed the existence of a novel cluster of the rumen Euryarchaeota, which is not affiliated with the known methanogenic archaea.