T
Takafumi Nagamine
Publications - 28
Citations - 1845
Takafumi Nagamine is an academic researcher. The author has contributed to research in topics: Prevotella ruminicola & Plasmid. The author has an hindex of 11, co-authored 28 publications receiving 1753 citations.
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Diet-dependent shifts in the bacterial population of the rumen revealed with real-time PCR.
TL;DR: The real-time PCR technique uncovered differential amplification of rumen bacterial templates with the set of universal bacterial primers, which may explain why some predominant rumen bacteria have not been detected in PCR-generated 16S ribosomal DNA libraries.
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Rumen bacterial diversity as determined by sequence analysis of 16S rDNA libraries
Kiyoshi Tajima,Roustam I. Aminov,Takafumi Nagamine,Koretsugu Ogata,Mutsumi Nakamura,Hiroki Matsui,Yoshimi Benno +6 more
TL;DR: The bacterial community structure was revealed by phylogenetic placement of sequences in relation to different fractions of rumen content and the vast majority of sequences from the rumen solids were found to be related to low G+C Gram-positive bacteria and the remaining sequences were placed within the Cytophaga-Flexibacter-Bacteroides and Spirochaetes phyla.
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Rumen Bacterial Community Transition During Adaptation to High-grain Diet
Kiyoshi Tajima,Shozo Arai,Koretsugu Ogata,Takafumi Nagamine,Hiroki Matsui,Mutsumi Nakamura,Rustam Aminov,Yoshimi Benno +7 more
TL;DR: Transitional changes of the ruminal bacterial community structure in cows during the switch from roughage to high-grain diet were monitored by PCR amplification and sequencing of 16S rDNA clone libraries.
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Phylogenetic analysis of archaeal 16S rRNA libraries from the rumen suggests the existence of a novel group of archaea not associated with known methanogens
TL;DR: Phylogenetic placement of eight almost complete 16S rRNA sequences revealed the existence of a novel cluster of the rumen Euryarchaeota, which is not affiliated with the known methanogenic archaea.
Journal ArticleDOI
Horizontal gene transfer from Bacteria to rumen Ciliates indicates adaptation to their anaerobic, carbohydrates-rich environment
Guenola Ricard,Neil R. McEwan,Bas E. Dutilh,Jean-Pierre Jouany,Didier Macheboeuf,Makoto Mitsumori,Freda M. McIntosh,Tadeusz Michałowski,Takafumi Nagamine,Nancy Nelson,Charles J. Newbold,Eli Nsabimana,Akio Takenaka,Nadine A. Thomas,Kazunari Ushida,Johannes H. P. Hackstein,Martijn A. Huynen +16 more
TL;DR: An over-representation (>75%) of genes involved in metabolism, specifically in the catabolism of complex carbohydrates, a rich food source in the rumen are found, providing evidence for the role of HGT in the adaptation to new niches.