Klaas Nico Faber

TL;DR: In contrast to electrotransformation protocols described for Saccharomyces cerevisiae and Candida maltosa, transformation frequencies for H. polymorpha remained high when large amounts of plasmid DNA were added, which renders this procedure pre-eminently advantageous for gene cloning experiments when high numbers of transformants are needed.

TL;DR: Overexpression of PER9 induced a significant increase in the number of peroxisomes per cell, a result that suggests that Per9p may be involved inperoxisome proliferation and/or membrane biosynthesis, and when PER9 expression was placed under the control of a strongly regulatable promoter and switched off, per oxisomes were observed to disintegrate over time in a manner that suggested that Per 9p may been required for maintenance of the peroxISomal membrane.

TL;DR: The potential of methylotrophic yeasts as hosts for the high level production of valuable foreign proteins and the possibility of accumulating the foreign gene products inside peroxisomes is discussed.

TL;DR: It is concluded that the peroxisomal targeting information of PTS2 is contained in the consensus sequence RL/I-X5-HL, which is present in the presequence of watermelon gMDH--namely, 10RI-X-17HL.

TL;DR: It is shown that a conserved stretch of positively charged amino acids in the N terminus of Hansenula polymorpha Pex3p is involved in incorporation of the protein into its target membrane.