The lion's share of bacteria in various environments cannot be cloned in the laboratory and thus cannot be sequenced using existing technologies. A major goal of single-cell genomics is to complement gene-centric metagenomic data with whole-genome assemblies of uncultivated organisms. Assembly of single-cell data is challenging because of highly non-uniform read coverage as well as elevated levels of sequencing errors and chimeric reads. We describe SPAdes, a new assembler for both single-cell and standard (multicell) assembly, and demonstrate that it improves on the recently released E+V-SC assembler (specialized for single-cell data) and on popular assemblers Velvet and SoapDeNovo (for multicell data). SPAdes generates single-cell assemblies, providing information about genomes of uncultivatable bacteria that vastly exceeds what may be obtained via traditional metagenomics studies. SPAdes is available online ( http://bioinf.spbau.ru/spades ). It is distributed as open source software.

SPAdes, a new genome assembly algorithm and its applications to single-cell sequencing ( 7th Annual SFAF Meeting, 2012)

https://opus.lib.uts.edu.au/bitstream/10453/33344/4/A%20review%20on%20the%20occurrence%20of%20micropollutants%20in%20the%20aquatic%20environment%20and%20their%20fate%20and%20removal%20during%20wastewater%20treatment.pdf

A review on the occurrence of micropollutants in the aquatic environment and their fate and removal during wastewater treatment

Removal of pharmaceuticals and personal care products (PPCPs) from wastewater: A review.

Occurrence and fate of emerging contaminants in municipal wastewater treatment plants from different geographical regions-a review.

Bisphenol A exposure, effects, and policy: A wildlife perspective

http://ceprofs.civil.tamu.edu/kchu/2009_Chemo_AOB_PPCPs.pdf

Biodegradation potential of wastewater micropollutants by ammonia-oxidizing bacteria.

Fourteen phylogenetically diverse 17β-estradiol-degrading bacteria (strains KC1−14) were isolated from activated sludge of a wastewater treatment plant. These isolates widely distributed among eight different generaAminobacter (strains KC6 and KC7), Brevundimonas (strain KC12), Escherichia (strain KC13), Flavobacterium (strain KC1), Microbacterium (strain KC5), Nocardioides (strain KC3), Rhodococcus (strain KC4), and Sphingomonas (strains KC8−KC11 and KC14)of three Phyla:  Proteobacteria, Actinobacteria, and Bacteroidetes. All 14 isolates were capable of converting 17β-estradiol to estrone, but only three strains (strains KC6, KC7, and KC8) showed the ability to degrade estrone. Only strain KC8 could use 17β-estradiol as a sole carbon source. Based on the degree of estrogens being transformed and the estrogenicity of metabolites and/or end products of estrogen degradation, three different degradation patterns (patterns A−C) were observed from degradation tests using resting cells. Eleven out of 14 isolate...

17β-estradiol-degrading bacteria isolated from activated sludge

Biodegradation of Potential Wastewater Micropollutants by Ammonia-oxidizing Bacteria

https://superfund.berkeley.edu/pdf/56.pdf

Kung-Hui Chu

Papers

Biodegradation potential of wastewater micropollutants by ammonia-oxidizing bacteria.

17β-estradiol-degrading bacteria isolated from activated sludge

Biodegradation of Potential Wastewater Micropollutants by Ammonia-oxidizing Bacteria

Variable carbon isotope fractionation expressed by aerobic CH4-oxidizing bacteria

Microbial degradation of steroidal estrogens