Kyle A. Anderson

TL;DR: It is shown that during PDI cooverexpression with the single-chain there is a sixfold increase in BiP levels, indicating that the former is possibly inducing an unfolded protein response due to excess chaperone and recombinant protein in the ER.

TL;DR: This study identifies proteins secreted or released into the culture media in the methanol-induced fermentation cultures of P. pastoris X-33 and suggests potential biotechnology applications based on the discovery of this proteome.

TL;DR: The results demonstrate that functional J591 ScFv can be produced in P. pastoris for use in diagnostic and targeted therapeutic applications and showed binding affinity and specificity to cell extracts containing PSMA and PSMA-expressing prostate cancer cells.

TL;DR: A process using current good manufacturing practices to produce clinical grade material was developed and executed to produce enough material for phase I clinical trials of NY‐ESO‐1, a cancer testis antigen expressed in numerous cancers.

TL;DR: Indirect immunofluorescence indicated that SSX2 containing the NLS did not translocate to the nucleus but accumulated in the endoplasmic reticulum (ER), and Experimental results suggested that SS X2 containingThe NLS was misfolded in the ER, while deletion of the N LS facilitated correct folding ofSSX2 inside the ER and improved its secretion.