L
Laura Ratier
Researcher at National University of General San Martín
Publications - 6
Citations - 294
Laura Ratier is an academic researcher from National University of General San Martín. The author has contributed to research in topics: Sialic acid & Trypanosoma cruzi. The author has an hindex of 6, co-authored 6 publications receiving 283 citations. Previous affiliations of Laura Ratier include National Scientific and Technical Research Council.
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Journal ArticleDOI
A sialidase mutant displaying trans-sialidase activity.
Gastón Paris,Laura Ratier,Maria Fernanda Amaya,T. Nguyen,Pedro M. Alzari,Alberto C.C. Frasch +5 more
TL;DR: The results show that the presence of a sugar acceptor binding-site, the fine-tuning of protein-substrate interactions and the flexibility of crucial active site residues are all important to achieve transglycosidase activity from the TrSA sialidase scaffold.
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Lactose derivatives are inhibitors of Trypanosoma cruzi trans-sialidase activity toward conventional substrates in vitro and in vivo.
TL;DR: The acceptor substrate specificity of lactose derivatives was studied by high pH anion-exchange chromatography with pulse amperometric detection and lactitol, which was the best of the ones tested, effectively inhibited the transfer of sialic acid to N-acetyllactosamine.
Journal ArticleDOI
Discovery of novel inhibitors of Trypanosoma cruzi trans-sialidase from in silico screening
João Neres,Mark L. Brewer,Laura Ratier,Horacio Botti,Alejandro Buschiazzo,Philip Neil Edwards,Paul N. Mortenson,Michael H. Charlton,Pedro M. Alzari,Alberto C.C. Frasch,Richard A. Bryce,Kenneth T. Douglas +11 more
TL;DR: Attempts to obtain crystal structures of these compounds with TcTS proved unsuccessful but provided evidence of ligand binding at the active site, including the 3-benzothiazol-2-yl-4-phenyl-but-3-enoic acid scaffold.
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Relevance of the diversity among members of the Trypanosoma cruzi trans-sialidase family analyzed with camelids single-domain antibodies.
Laura Ratier,Mariela Urrutia,Gastón Paris,Laura Zarebski,Alberto C.C. Frasch,Fernando Alberto Goldbaum +5 more
TL;DR: The results suggest that the presence of a large and diverse trans-sialidase family might be required to prevent the inhibitory response against this essential enzyme and might thus constitute a novel strategy of T. cruzi to evade the host immune system.
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Synthesis of PEGylated lactose analogs for inhibition studies on T.cruzi trans-sialidase
TL;DR: In this paper, a covalent conjugation of polyethylene glycol (PEG) with lactose, lactobionolactone and benzyl β-D-galactopyranosyl- (1→6)-2-amino-2-deoxy-α-Dglucopyranoide (1) with the hope of improving the bioavailability, though retaining their inhibitory properties.