Laurent Magnenat

TL;DR: It is reported that interleukin 23 and the transcription factor RORγt drove expression of the cytokine GM-CSF in helper T cells, whereas IL-12, interferon-γ (IFN-γ) and IL-27 acted as negative regulators.

TL;DR: This report is the first report of target site selection for designed, well characterized 6-finger proteins and has important implications for the design of proteins that can recognize extended DNA sequences, as well as provide insights into the general rules of recognition for naturally occurring zinc finger proteins.

TL;DR: Large combinatorial libraries of artificial transcription factors comprising three or six zinc-finger domains are prepared, and selected transcription factor–DNA interactions able to upregulate several genes in human cells are selected.

TL;DR: These transcriptional activators constitute a novel class of regulators of the globin locus that may be suitable for treatment of diseases arising from mutations in this locus such as sickle cell disease and thalassemic diseases.

TL;DR: Combining library and affinity maturation approaches generated superior TFZFs that may find further applications in therapeutic research and in ICAM-1 biology, and also provided novel mechanistic insights into the biology of transcription factors.