Letícia Ferrari Crocomo

TL;DR: Using only 2.5% FCS and the addition of PES from Day 4, increased the survival of IVP embryos after vitrification, and embryo quality, represented by the fresh apoptosis rate, was better than lipid content for predicting embryo survival after vitrified embryos.

TL;DR: It was concluded that forskolin is an effective lipolytic agent even at low concentrations, leading to formation of blastocysts with a comparatively larger number of cells.

TL;DR: The results of this experiment indicated that the omission of FCS and the addition of forskolin do not have deleterious effect on embryo production rates and embryos produced in the presence of F CS had greater sensitivity to cryopreservation, but this effect was reversed when forSkolin was added to the medium, which improved embryo survival without affecting embryo development and quality after vitrification.

TL;DR: The increased lipid content was moderately correlated with apoptosis in vitrified blastocysts, which indicated that the apoptosis rate in fresh embryos was a better parameter than the lipid content to predict post-vitrification embryo survival.

TL;DR: It is demonstrated that roscovitine promoted irreversible ultrastructural changes while cycloheximide did not affect the cytoplasmic maturation, however, the implications on embryo development are still unclear.