Showing papers by "Lewis L. Lanier published in 1983"

Subpopulations of human natural killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NK-15) antigens.

Abstract: The functional and phenotypic characteristics of cells in human peripheral blood that mediate "natural killer" (NK) cytolysis have been examined with the use of multiparameter flow cytometry analysis and cell sorting. Essentially, all lymphocytes expressing NK and ADCC activity reacted with the anti-Leu-11a monoclonal antibody. The Leu-11a antigen was expressed on cytotoxic large granular lymphocytes (LGL), neutrophils, and basophils, but was not present on B cells, mitogen-activated T lymphoblasts, or Leu-1+ and Leu 4+ resting T cells. Anti-Leu-11a antibody selectively inhibited the binding of FITC heat-aggregated IgG complexes to granulocytes and LGL, and it may recognize a type of Fc receptor on these cells. Two-color FACS cell sorting indicated the existence of four lymphocyte subsets defined by the expression of Leu-11a and Leu-7 antigens. The Leu-11a+, -7- cells were highly active in 4-hr NK assays with the use of 51Cr-labeled K562 as the target. In contrast, the Leu-11a-, -7+ cells demonstrated weak activity and the Leu-11a-, -7- cells demonstrated no activity. The function of the Leu 11a+, -7+ cells varied considerably among several individuals examined. Multiparameter analysis with the use of two-color flow cytometry was used to determine the relationship between the expression of these NK-associated antigens and T and B cell-associated markers. These data indicate that considerable heterogeneity exists within human peripheral lymphocytes with regard to cell phenotype and function, but that several defined cellular subsets can be clearly revealed by using multiparameter FACS analysis and sorting.

Correlation of functional properties of human lymphoid cell subsets and surface marker phenotypes using multiparameter analysis and flow cytometry.

Abstract: INTRODUCTION Recognition of heterogeneity in lymphocyte populations has been achieved both through the discovery of selective expression of specific cell surface antigens on lymphoid cells and by findings of different functional properties of cells bearing these antigens. The explosive development of hybridoma technology, coupled with the analytical and separation capabilities of fluorescence-activated cell sorting, has led to an extensive classification of human lymphocytes into defined subsets. Of particular importance is the fact that expression of certain cell surface antigens correlates with functional properties or the differentiation state of the cell. Although in most cases the actual physiological role of the cell surface antigen is as yet unknown, monoclonal antibodies can be used to positively or negatively select identified subpopulations of cells for further functional, biochemical or genetic studies. It is anticipated that eventually it should be possible to unequivocally identify only those cells mediating a specific and select function by analysis of their cell surface phenotype.

Correlation of biophysical properties and cell surface antigenic profile of Percoll gradient-separated human natural killer cells.

Abstract: Human peripheral blood nylon wool nonadherent lymphocytes were fractionated according to cellular density on discontinuous Percoll gradients. The various fractions of cells obtained by this procedure were then analyzed to correlate morphology, cytotoxicity, light scatter properties and antigenic profile. The majority of natural killer (NK) cell activity was manifested by light density lymphocytes, which demonstrated relatively high light scatter characteristics, large granular lymphocyte morphology, and expressed the phenotypes: Leu 11+(B73.1+), Leu 7-; Leu 11+(B73.1+), Leu 7+, and Leu 11+(B73.1+), Leu 2a+ (low density), Leu 4-. The denser fractions of Percoll (fractions 3-4) tended to show lower NK activity, fewer large granular lymphocytes, lower light scatter profiles, and to selectively localize the subpopulations of lymphocytes with the phenotypes: Leu 11-, Leu 7+, Leu 4+, and Leu 11-, Leu 7+, Leu 2a+ (high density).

Differentiation of natural killer cell subpopulations of cells

Abstract: A method for distinguishing multiple subpopulations of a cell sample whereby human natural killer cells subpopulations can be monitored. The method utilizes two monoclonal antibodies identified as anti-Leu-7 and anti-Leu-11.

Method for monitoring activated cell subpopulations

Abstract: A method of distinguishing multiple subpopulations of a cell sample whereby resting and activated human natural killer cells subpopulations can be monitored. This method utilizes two monoclonal antibodies identified as anti-Leu 11 and anti-DR (or anti-Leu 10 or anti-transferrin receptor).

Lym 7.3: New murine specificity defining third allele of the ly 7 locus.

Abstract: A new antigenic specificity, designated Lym 7.3, defines a third allele of the Ly 7 locus. The monoclonal antibody recognizing this determinant was produced by a hybridoma derived from immunization of B10.D2 Ign mice against a (BALB/c x NZB) F1 murine B lymphoma cell line (WEHI-5). Previously, we reported that another antibody (BD5-334.5) from this fusion reacted with an alloantigen similar or identical to the Ly 7.2 antigen originally described by McKenzie and coworkers. The Lym 7.3 specificity is qualitatively and quantitatively identical to the Lym 7.2 determinant in tissue distribution. Both antibodies react with a majority of splenocytes, peripheral blood lymphocytes, lymph node cells, and mitogen activated blasts from positive strains, but do not react with kidney, liver, or brain tissues by absorption analysis. B lymphocytes expressed significantly higher cell surface density of Lym 7.2 and Lym 7.3 than T lymphocytes. However, these antibodies clearly could be distinguished on the basis of inbred a...

Hematopoietic Tumors: Normal or Abnormal Models of Leukocyte Differentiation?

Abstract: One of the major problems in cellular immunobiology has been the issue of resolving the cellular bases of the many different specific functional properties mediated by the immune system. This is in essence a dual problem, as it involves both a detailed phenotypic characterization of all the differentiation lineages in the hematopoietic system, and the correlation of each cellular subset with specific functional properties. This problem is particularly compounded both by the wide heterogeneity in the differentiation stages of cells within hematopoietic tissues, and by the requirements for collaboration and interaction amongst different cell types in the mediation of specific immunological functions (Katz and Benacerraf, 1977). Analysis of the cell types involved in any given immune response is further complicated by the wide heterogeneity in cell populations when cell suspensions are obtained from in vivo tissue and blood sources.