비만아 부모의 돌봄 경험: q 방법론

Micromachining technology was used to prepare chemical analysis systems on glass chips (1 centimeter by 2 centimeters or larger) that utilize electroosmotic pumping to drive fluid flow and electrophoretic separation to distinguish sample components. Capillaries 1 to 10 centimeters long etched in the glass (cross section, 10 micrometers by 30 micrometers) allow for capillary electrophoresis-based separations of amino acids with up to 75,000 theoretical plates in about 15 seconds, and separations of about 600 plates can be effected within 4 seconds. Sample treatment steps within a manifold of intersecting capillaries were demonstrated for a simple sample dilution process. Manipulation of the applied voltages controlled the directions of fluid flow within the manifold. The principles demonstrated in this study can be used to develop a miniaturized system for sample handling and separation with no moving parts.

Micromachining a miniaturized capillary electrophoresis-based chemical analysis system on a chip

Owing to its high carrier mobility, conductivity, flexibility and optical transparency, graphene is a versatile material in micro- and macroelectronics. However, the low density of electrochemically active defects in graphene synthesized by chemical vapour deposition limits its application in biosensing. Here, we show that graphene doped with gold and combined with a gold mesh has improved electrochemical activity over bare graphene, sufficient to form a wearable patch for sweat-based diabetes monitoring and feedback therapy. The stretchable device features a serpentine bilayer of gold mesh and gold-doped graphene that forms an efficient electrochemical interface for the stable transfer of electrical signals. The patch consists of a heater, temperature, humidity, glucose and pH sensors and polymeric microneedles that can be thermally activated to deliver drugs transcutaneously. We show that the patch can be thermally actuated to deliver Metformin and reduce blood glucose levels in diabetic mice.

A graphene-based electrochemical device with thermoresponsive microneedles for diabetes monitoring and therapy

Capillarity and Wetting Phenomena: Drops, Bubbles, Pearls, Waves

The light microscope is traditionally an instrument of substantial size and expense. Its miniaturized integration would enable many new applications based on mass-producible, tiny microscopes. Key prospective usages include brain imaging in behaving animals for relating cellular dynamics to animal behavior. Here we introduce a miniature (1.9 g) integrated fluorescence microscope made from mass-producible parts, including a semiconductor light source and sensor. This device enables high-speed cellular imaging across ∼0.5 mm2 areas in active mice. This capability allowed concurrent tracking of Ca2+ spiking in >200 Purkinje neurons across nine cerebellar microzones. During mouse locomotion, individual microzones exhibited large-scale, synchronized Ca2+ spiking. This is a mesoscopic neural dynamic missed by prior techniques for studying the brain at other length scales. Overall, the integrated microscope is a potentially transformative technology that permits distribution to many animals and enables diverse usages, such as portable diagnostics or microscope arrays for large-scale screens.

/pdf/miniaturized-integration-of-a-fluorescence-microscope-57zyni1k2h.pdf

Miniaturized integration of a fluorescence microscope

The skin, commonly considered to be the largest organ of the human body, has been used as a drug delivery route for numerous dermal and transdermal drugs. Being a structural barrier protecting the underlying tissues, the skin poses many challenges to be used as an amenable passage for drugs to permeate. In this chapter, we would describe the current advantages and disadvantages of transdermal drug delivery, making comparisons with different drug delivery routes, elucidate the mechanisms of passive and active transdermal drug delivery and deliberating different formulations which can be delivered via the transdermal route. We would investigate the current status and the development of microneedles, a strategy invented to transiently breach the skin’s stratum corneum to deliver drugs through the skin. We would also investigate the different types of microneedles, provide insights into the microneedles used in clinical trials and diffusion cell systems used to assess the efficacy of microneedles in their capacity to deliver drugs through the skin.

Introduction & Literature Review

Background Curcumin (CUR) is a functional ingredient from the spice turmeric. It has attracted considerable attention recently, owing to its diverse biological activities. However, curcumin has low water solubility, which limited its applications. Some sugar molecules were found to be able to solubilise poorly water-soluble compounds by forming micelles in aqueous solutions. Purpose To improve the water solubility and oral absorption of CUR, using a non-nutritive natural sweetener, namely, Mogroside V (Mog-V). Methods A solid dispersion of CUR in Mog-V was prepared using a solvent evaporation method. The solid dispersion was characterised by using X-ray diffraction and differential scanning calorimetry. The solid dispersion can dissolve in water to form micelles with a diameter of ~160 nm, which were characterised by using dynamic light scattering. To find out the mechanism of solubilisation, the aggregation behaviour of Mog-V molecules in aqueous solution was investigated using nuclear magnetic resonance spectroscopy. Finally, oral absorption of CUR in the solid dispersion was evaluated using a rodent model. Results A solid dispersion was formed in a ratio of 1 CUR to 10 Mog-V by weight. Upon dissolution into water, CUR laden micelles formed via self-assembly of Mog-V molecules, which increased the solubility of CUR by nearly 6000 times compared with pure CUR crystals. In rats, the solid dispersion increased the oral absorption of CUR by 29 folds, compared with CUR crystals. In terms of solubilisation mechanism, it was found that Mog-V self-assembled into micelles with a core-shell structure and CUR molecules were incorporated into the hydrophobic core of the Mog-V micelles. Conclusion Mog-V can form a solid dispersion with CUR. Upon dissolution in water, the Mog-V in the solid dispersion can self-assemble into micelles, which solubilise CUR and increase its oral absorption.

Solubilisation and Enhanced Oral Absorption of Curcumin Using a Natural Non-Nutritive Sweetener Mogroside V

A new mathematical method is established to represent the operator, wave functions and square matrix in the same representation. We can obtain the specific square matrices corresponding to the angular momentum and Runge-Lenz vector operators with invoking assistance from the operator relations and the orthonormal wave functions. Furthermore, the first-order differential equations will be given to deduce the specific wave functions without using the solution of the second order Schrodinger equation. As a result, we will unify the descriptions of the matrix mechanics and the wave mechanics on hydrogen atom. By using matrix transformations, we will also deduce the specific matrix representations of the operators in the SO (4,2) group.

Unified Description of Matrix Mechanics and Wave Mechanics on Hydrogen Atom

Development and validation of a highly sensitive LC-MS/MS method for determination of brain active agent dianhydrogalactitol in mouse plasma and tissues: Application to a pharmacokinetic study.

In this article, we discard the bra-ket notation and its correlative definitions, given by Paul Dirac. The quantum states are only described by the wave functions. The fundamental concepts and definitions in quantum mechanics is simplified. The operator, wave functions and square matrix are represented in the same expression which directly corresponds to the system of equations without additional introduction of the matrix representation of operator. It can make us to convert the operator relations into the matrix relations. According to the relations between the matrices, the matrix elements will be determined. Furthermore, the first order differential equations will be given to find the solution of equations. As a result, we unified the descriptions of the matrix mechanics and the wave mechanics.

Lifeng Kang

Papers

Introduction & Literature Review

Solubilisation and Enhanced Oral Absorption of Curcumin Using a Natural Non-Nutritive Sweetener Mogroside V

Unified Description of Matrix Mechanics and Wave Mechanics on Hydrogen Atom

Development and validation of a highly sensitive LC-MS/MS method for determination of brain active agent dianhydrogalactitol in mouse plasma and tissues: Application to a pharmacokinetic study.

Unified Description of Matrix Mechanics and Wave Mechanics I