Luyan Ma

TL;DR: Direct visualization reveals that Psl is a key scaffolding matrix component and opens up avenues for therapeutics of biofilm-related complications.

TL;DR: Analysis of biofilms formed by the Deltapsl/p(BAD)-psl strain indicated that expression of the psl operon is required to maintain the biofilm structure at steps postattachment, which is critical for initiation and maintenance of theBiofilm structure.

TL;DR: Chemical composition analyses and mannose- or galactose-specific lectin staining are used, followed by confocal laser scanning microscopy and electron microscopy, to show that Psl is a galactOSE-rich and manNose-rich exopolysaccharide.

TL;DR: It is demonstrated that TAGE does not have selective toxicity against cells within the biofilm state, will inhibit biofilm development under flow conditions, indicating that the CV staining protocol correlates with the ability to be active under biomimetic conditions, and will disperse preformed P. aeruginosa biofilms.

TL;DR: The site within MinE that is required for interaction with MinD was mapped by studying the effects of site-directed minE mutations on MinD-MinE interactions in yeast two-hybrids and three-hybrid experiments, confirming that the MinE N-terminal domain is responsible for the interaction of MinE with Min D.