M. J. Yezzi

TL;DR: The successful establishment of a postcrisis SV40 large T-antigen transformed epithelial cell line derived from human bronchial epithelium is described, and this cell line, 16HBE14o- cells, provides a valuable resource for studying the modulation of CFTR and its role in regulation of chloride ion transport in human airway epithelia as well as other aspects of human airways cell biology.

TL;DR: These cells demonstrate that it is possible to select transformed cell clones with particular morphologic characteristics, i.e. the presence of tight junctions and cell polarity, which also retain useful epithelial cell-specific functions, including vectorial ion transport.

TL;DR: Cl- transport measured by 36Cl efflux shows that CF gland epithelial cells, like CF surface airway and nasal polyp epithel cells, are unable to respond to increases in intracellular cAMP, however, they do produce an increase in intrusion cAMP after treatment with isoproterenol or forskolin.

TL;DR: This work has employed Epstein-Barr virus (EBV)-based expression vectors for correction of the cAMP-dependent Cl- transport defect associated with CF by introducing a CFTR-containing expression construct under the regulation of the Rous sarcoma virus (RSV) long terminal repeat (LTR).

TL;DR: A selection protocol that involves growing cells in medium that is high in Ca2+ and supplemented with fetal bovine serum (FBS) has been employed to facilitate progression through crisis and one cell line that has a normal phenotype and retains tight junctions post-crisis has been produced.