Summary
The genetic and molecular basis of biofilm formation in staphylococci is multifaceted. The ability to form a biofilm affords at least two properties: the adherence of cells to a surface and accumulation to form multilayered cell clusters. A trademark is the production of the slime substance PIA, a polysaccharide composed of β-1,6-linked N-acetylglucosamines with partly deacetylated residues, in which the cells are embedded and protected against the host’s immune defence and antibiotic treatment. Mutations in the corresponding biosynthesis genes (ica operon) lead to a pleiotropic phenotype; the cells are biofilm and haemagglutination negative, less virulent and less adhesive on hydrophilic surfaces. ica expression is modulated by various environmental conditions, appears to be controlled by SigB and can be turned on and off by insertion sequence (IS) elements. A number of biofilm-negative mutants have been isolated in which polysaccharide intercellular adhesin (PIA) production appears to be unaffected. Two of the characterized mutants are affected in the major autolysin (atlE) and in D-alanine esterification of teichoic acids (dltA). Proteins have been identified that are also involved in biofilm formation, such as the accumulation-associated protein (AAP), the clumping factor A (ClfA), the staphylococcal surface protein (SSP1) and the biofilm-associated protein (Bap). Concepts for the prevention of obstinate polymer-associated infections include the search for new anti-infectives active in biofilms and new biocompatible materials that complicate biofilm formation and the development of vaccines.

https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2958.2002.02827.x

Staphylococcus and biofilms.

Bioceramics of calcium orthophosphates

The concept of producing 'spare parts' of the body for replacement of damaged or lost organs lies at the core of the varied biotechnological practices referred to generally as tissue engineering. Use of postnatal stem cells has the potential to significantly alter the perspective of tissue engineering. Successful long-term restoration of continuously self-renewing tissues such as skin, for example, depends on the use of extensively self-renewing stem cells. The identification and isolation of stem cells from a number of tissues provides appropriate targets for prospective gene therapies.

Stem cells in tissue engineering.

Silver nanoparticles can be coated on common polyurethane (PU) foams by overnight exposure of the foams to nanoparticle solutions. Repeated washing and air-drying yields uniformly coated PU foam, which can be used as a drinking water filter where bacterial contami- nation of the surface water is a health risk. Nanoparticles are stable on the foam and are not washed away by water. Morphology of the foam was retained after coating. The nanoparticle binding is due to its interaction with the ni- trogen atom of the PU. Online tests were conducted with a prototypical water filter. At a flow rate of 0.5 L/min, in which contact time was of the order of a second, the output count of Escherichia coli was nil when the input water had a bacterial load of 10 5 colony-forming units (CFU) per mL. Combined with the low cost and effectiveness in its applications, the technology may have large implications to developing countries. B 2005 Wiley Periodicals, Inc.

https://www.dstuns.iitm.ac.in/listpdf/132.pdf

Potential of silver nanoparticle-coated polyurethane foam as an antibacterial water filter

The present overview is intended to point the readers’ attention to the important subject of calcium orthophosphates. These materials are of the special significance because they represent the inorganic part of major normal (bones, teeth and dear antlers) and pathological ( i.e. those appearing due to various diseases) calcified tissues of mammals. Due to a great chemical similarity with the biological calcified tissues, many calcium orthophosphates possess remarkable biocompatibility and bioactivity. Materials scientists use this property extensively to construct artificial bone grafts that are either entirely made of or only surface-coated with the biologically relevant calcium ortho-phosphates. For example, self-setting hydraulic cements made of calcium orthophosphates are helpful in bone repair, while titanium substitutes covered by a surface layer of calcium orthophosphates are used for hip joint endoprostheses and as tooth substitutes. Porous scaffolds made of calcium orthophosphates are very promising tools for tissue engineering applications. In addition, technical grade calcium orthophosphates are very popular mineral fertilizers. Thus ere calcium orthophosphates are of great significance for humankind and, in this paper, an overview on the current knowledge on this subject is provided.

https://www.mdpi.com/1996-1944/2/2/399/pdf

Calcium Orthophosphates in Nature, Biology and Medicine

Resorbable bioceramics based on stabilized calcium phosphates. Part II: evaluation of biological response.

Structure and composition of silicon-stabilized tricalcium phosphate

Resorbable bioceramics based on stabilized calcium phosphates. Part I: rational design, sample preparation and material characterization

Phase formation and evolution in the silicon substituted tricalcium phosphate/apatite system.

The antimicrobial activity of Ag, Cu, and layered Ag–Cu surface films, sputter-coated onto several types of catheter material, against clinical isolates of Staphylococcus epidermidis and Staphyloco...

M. Sayer

Papers

Resorbable bioceramics based on stabilized calcium phosphates. Part II: evaluation of biological response.

Structure and composition of silicon-stabilized tricalcium phosphate

Resorbable bioceramics based on stabilized calcium phosphates. Part I: rational design, sample preparation and material characterization

Phase formation and evolution in the silicon substituted tricalcium phosphate/apatite system.

Antibacterial activity of multilayer silver–copper surface films on catheter material