Mai-Liis Org

TL;DR: A quantitative analysis of transfer efficiencies of building blocks, as they are used for typical 3D experiments, was performed and six experiments were selected to optimally assign the model protein ubiquitin, solely using spectra acquired at 93 kHz MAS.

TL;DR: A predominantly linear improvement in linewidths and coherence decay times of protons with increasing spinning frequency in the range from 93 to 126 kHz is found.

TL;DR: It is concluded that even faster MAS is highly attractive and increases mass sensitivity at a moderate price in overall sensitivity.

TL;DR: It is demonstrated that 1H and 15N relaxation-based sPREs provide a powerful tool for characterizing intermolecular interactions in large assemblies in the solid state and are used to characterize protein–protein interfaces in the GB1 complex with immunoglobulin G.

TL;DR: A new class of frequency-selective homonuclear recoupling methods to selectively enhance 1H-1H correlations of interest under ultrafast magic-angle-spinning (MAS), dubbed as Selective Phase-optimized Recoupling (SPR), can provide a sensitivity enhancement by a factor of ~ 3 over the widely-used radio-frequency driven recoupled (RFDR).