Protein NMR Spectroscopy at 150 kHz Magic-Angle Spinning Continues To Improve Resolution and Mass Sensitivity
Maarten Schledorn,Alexander A. Malär,Anahit Torosyan,Susanne Penzel,Daniel Klose,Andres Oss,Mai-Liis Org,Shishan Wang,Lauriane Lecoq,Riccardo Cadalbert,Ago Samoson,Anja Böckmann,Beat H. Meier +12 more
TLDR
It is concluded that even faster MAS is highly attractive and increases mass sensitivity at a moderate price in overall sensitivity.Abstract:
Spectral resolution is the key to unleashing the structural and dynamic information contained in NMR spectra. Fast magic-angle spinning (MAS) has recently revolutionized the spectroscopy of biomolecular solids. Herein, we report a further remarkable improvement in the resolution of the spectra of four fully protonated proteins and a small drug molecule by pushing the MAS rotation frequency higher (150 kHz) than the more routinely used 100 kHz. We observed a reduction in the average homogeneous linewidth by a factor of 1.5 and a decrease in the observed linewidth by a factor 1.25. We conclude that even faster MAS is highly attractive and increases mass sensitivity at a moderate price in overall sensitivity.read more
Citations
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Structure of Fully Protonated Proteins by Proton-Detected Magic-Angle Spinning NMR
Loren B. Andreas,Kristaps Jaudzems,Jan Stanek,Daniela Lalli,Andrea Bertarello,T. Le Marchand,D. Cala-De Paepe,Svetlana Kotelovica,Inara Akopjana,Benno Knott,Sebastian Wegner,Frank Engelke,A. Lesage,Lyndon Emsley,Kaspars Tars,Torsten Herrmann,Guido Pintacuda +16 more
TL;DR: In this article, the authors used magic-angle spinning (MAS) to detect resolved 1H resonances in protonated proteins by increasing MAS rates to frequencies of 100 kHz and above.
Journal ArticleDOI
Solid-State NMR for Studying the Structure and Dynamics of Viral Assemblies
TL;DR: The way from first solid-state NMR investigations to the state-of-the-art approaches currently developing is traced, including applications focused on HIV, HBV, HCV and influenza, and an outlook to the possibilities opening in the coming years.
Journal ArticleDOI
Proton Detected Solid-State NMR of Membrane Proteins at 28 Tesla (1.2 GHz) and 100 kHz Magic-Angle Spinning.
Evgeny Nimerovsky,Kumar Tekwani Movellan,Xizhou Cecily Zhang,Marcel C. Forster,Eszter E. Najbauer,Kai Xue,Rıza Dervişoǧlu,Karin Giller,Christian Griesinger,Stefan Becker,Loren B. Andreas +10 more
TL;DR: The available magnetic field strength for high resolution NMR in persistent superconducting magnets has recently improved from 23.5 to 28 Tesla, increasing the proton resonance frequency from 1 to 1.2 GHz as mentioned in this paper.
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Biomolecular solid-state NMR spectroscopy at 1200 MHz: the gain in resolution
Morgane Callon,Alexander A. Malär,Sara Pfister,Václav Římal,Marco E. Weber,Thomas Wiegand,Johannes Zehnder,Matías Chávez,Riccardo Cadalbert,Rajdeep Deb,Alexander Däpp,Marie-Laure Fogeron,Andreas Hunkeler,Lauriane Lecoq,Anahit Torosyan,Dawid Zyla,Rudolf Glockshuber,Stefanie Jonas,Michael Nassal,Matthias Ernst,Anja Böckmann,Beat H. Meier +21 more
TL;DR: In this paper, a collection of high-field NMR spectra of a variety of proteins, including molecular machines, membrane proteins, viral capsids, fibrils and large molecular assemblies, are presented.
Journal ArticleDOI
1H-Detected Biomolecular NMR under Fast Magic-Angle Spinning
Tanguy Le Marchand,Tobias Schubeis,Marta Bonaccorsi,Piotr Paluch,Daniela Lalli,Andrew J. Pell,Loren B. Andreas,Kristaps Jaudzems,Jan Stanek,Guido Pintacuda +9 more
TL;DR: The many examples where 1H-detected MAS NMR has contributed to the detailed characterization of a variety of crystalline and noncrystalline biomolecular targets involved in biological processes ranging from catalysis through drug binding, viral infectivity, amyloid fibril formation, to transport across lipid membranes are outlined.
References
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