M
Manabu Shimonishi
Researcher at Toyobo
Publications - 15
Citations - 3260
Manabu Shimonishi is an academic researcher from Toyobo. The author has contributed to research in topics: Complementary DNA & Recombinant DNA. The author has an hindex of 8, co-authored 15 publications receiving 3206 citations. Previous affiliations of Manabu Shimonishi include Kyushu University.
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Journal ArticleDOI
Molecular cloning and expression of human hepatocyte growth factor
Toshikazu Nakamura,Tsutomu Nishizawa,Mitchio Hagiya,Tatsuya Seki,Manabu Shimonishi,Atsushi Sugimura,Kosuke Tashiro,Shin Shimizu +7 more
TL;DR: The nucleotide sequence of the human HGF cDNA reveals that both α- andβ-chains are contained in a single open reading frame coding for a pre-pro precursor protein of 728 amino acids, which indicates that the activity of HGF is not species-specific.
Journal ArticleDOI
Deduced primary structure of rat hepatocyte growth factor and expression of the mRNA in rat tissues.
Kosuke Tashiro,Mitchio Hagiya,Tsutomu Nishizawa,Tatsuya Seki,Manabu Shimonishi,Shin Shimizu,Toshikazu Nakamura +6 more
TL;DR: The primary structure of rat hepatocyte growth factor (HGF) was elucidated by determining the base sequence of the complementary DNA (cDNA) of HGF by screening a liver cDNA library with oligonucleotides based on the partial N-terminal amino acid sequence ofthe beta subunit of purified rat HGF.
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Isolation and expression of cDNA for different forms of hepatocyte growth factor from human leukocyte.
Tatsuya Seki,Tatsuya Seki,Izumi Ihara,Izumi Ihara,Atsushi Sugimura,Atsushi Sugimura,Manabu Shimonishi,Manabu Shimonishi,Tsutomu Nishizawa,Tsutomu Nishizawa,Osamu Asami,Osamu Asami,Hagiya Michio,Hagiya Michio,Toshikazu Nakamura,Toshikazu Nakamura,Shin Shimizu,Shin Shimizu +17 more
TL;DR: Human leukocyte cDNA library was screened to isolate cDNA clones coding for hepatocyte growth factor using cDNA from human liver as a probe and both clones produced protein with similar biological activity against rat hepatocyte in vitro.
Journal ArticleDOI
Cloning and sequence analysis of the rat augmenter of liver regeneration (ALR) gene: expression of biologically active recombinant ALR and demonstration of tissue distribution.
Michio Hagiya,Antonio Francavilla,L Polimeno,Izumi Ihara,Harumi Sakai,Tatsuya Seki,Manabu Shimonishi,Kendrick A. Porter,Thomas E. Starzl +8 more
TL;DR: The 125-aa sequence deduced from the rat ALR cDNA shows 50% homology to the amino acid sequence of the gene for oxidative phosphorylation and vegetative growth in the yeast Saccharomyces cerevisiae.
Journal ArticleDOI
Organization of the human hepatocyte growth factor-encoding gene.
TL;DR: The organization of the HGF gene is highly homologous to those of the serine proteases involved in blood coagulation and fibrinolysis, especially with that of plasminogen, which suggests that the human H GF gene is evolutionally related to these genes.