Mani Subramanian

TL;DR: This work shows the first example of direct conversion of theophylline to 3-methylxanthine by a metabolically engineered strain of E. coli, and represents a first step to develop a new biological platform for the production of methylxanthines from economical feedstocks such as caffeine, theobromine, and theophyLLine.

TL;DR: It is shown that the addicted strain can be used as a biosensor to measure the caffeine content of common beverages and could be useful for reclaiming nutrient-rich byproducts of coffee bean processing and for the cost-effective bioproduction of methylxanthine drugs.

TL;DR: Preliminary results for production of theobromine and 7-methylxanthine from caffeine and theOBromine, respectively, by two strains of metabolically engineered E. coli are presented and complete decaffeination of tea extract is demonstrated by an immobilized mixed culture of Klebsiella and Rhodococcus cells.

TL;DR: In this article, a two-stage strategy was proposed to produce bio-xylitol from d -xylose using a recombinant Pichia pastoris expressing a heterologous xylose reductase gene.

TL;DR: This is the first report of theobromine production via a metabolically engineered strain, with the highest yield and purity reported of any biological method, and eliminates the harsh reaction conditions and environmental issues associated with the chemical process.