M
Manju Varma
Researcher at United States Environmental Protection Agency
Publications - 30
Citations - 2094
Manju Varma is an academic researcher from United States Environmental Protection Agency. The author has contributed to research in topics: Bacteroidales & Indicator bacteria. The author has an hindex of 19, co-authored 29 publications receiving 1934 citations.
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Journal ArticleDOI
Quantitative PCR Analysis of Selected Aspergillus, Penicillium and Paecilomyces Species
TL;DR: Evidence is provided that the commonly used practices of enumerating Aspergillus and Penicillium as a single group or only by genus can be misleading in understanding the indoor populations of these organisms and their potential health risks.
Journal ArticleDOI
Improved HF183 quantitative real-time PCR assay for characterization of human fecal pollution in ambient surface water samples
Hyatt C. Green,Richard A. Haugland,Manju Varma,Hana T. Millen,Mark A. Borchardt,Katharine G. Field,William A. Walters,Rob Knight,Rob Knight,Mano Sivaganesan,Catherine A. Kelty,Orin C. Shanks +11 more
TL;DR: The new TaqMan HF183/BacR287 assay should provide more accurate estimations of human-derived fecal contaminants in ambient surface waters.
Journal ArticleDOI
Evaluation of genetic markers from the 16S rRNA gene V2 region for use in quantitative detection of selected Bacteroidales species and human fecal waste by qPCR.
Richard A. Haugland,Manju Varma,Mano Sivaganesan,Catherine A. Kelty,Lindsay Peed,Orin C. Shanks +5 more
TL;DR: Analysis for multiple individual Bacteroidales species may be useful in identifying human fecal pollution in environmental waters, as suggested by results of this study.
Journal ArticleDOI
Quantitative PCR for Genetic Markers of Human Fecal Pollution
TL;DR: Qualitative PCR assays for quantification of two recently described human-specific genetic markers targeting Bacteroidales-like cell surface-associated genes are reported, providing experimental evidence supporting the potential application of these quantitative methods for monitoring fecal pollution in ambient environmental waters.
Journal ArticleDOI
Quantitative real-time PCR analysis of total and propidium monoazide-resistant fecal indicator bacteria in wastewater
TL;DR: Reductions of PMA-qPCR as well as total qPCR detectable target sequences from enterococci and, to a lesser extent, Bacteroidales correlated well with reductions in infectious viruses during both normal and storm flow operating conditions and therefore may have predictive value in determining the efficiency at which these pathogens are removed.