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Orin C. Shanks
Researcher at United States Environmental Protection Agency
Publications - Â 89
Citations - Â 4888
Orin C. Shanks is an academic researcher from United States Environmental Protection Agency. The author has contributed to research in topics: Indicator bacteria & Bacteroidales. The author has an hindex of 39, co-authored 81 publications receiving 4150 citations. Previous affiliations of Orin C. Shanks include Oregon State University.
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Journal ArticleDOI
Community structures of fecal bacteria in cattle from different animal feeding operations.
Orin C. Shanks,Catherine A. Kelty,S. L. Archibeque,Michael B. Jenkins,Ryan J. Newton,Sandra L. McLellan,Susan M. Huse,Mitchell L. Sogin +7 more
TL;DR: Network analysis demonstrated that annotated sequences clustered by management practice and fecal starch concentration, suggesting that the structures of bovine fecal bacterial communities can be dramatically different in different animal feeding operations, even at the phylum and family taxonomic levels.
Journal ArticleDOI
Performance of forty-one microbial source tracking methods: a twenty-seven lab evaluation study.
Alexandria B. Boehm,Laurie C. Van De Werfhorst,John F. Griffith,Patricia A. Holden,Jenny Jay,Orin C. Shanks,Dan Wang,Stephen B. Weisberg +7 more
TL;DR: The most sensitive and specific MST methodologies, based on an analysis of presence/absence of each marker in target and non-target fecal samples, were HF183 endpoint and HF183SYBR (human), CF193 and Rum2Bac (ruminant), CowM2 and CowM3 (cow), BacCan (dog), Gull2SYBR and LeeSeaGull (gull), and Pig2B Bac (pig).
Journal ArticleDOI
Improved HF183 quantitative real-time PCR assay for characterization of human fecal pollution in ambient surface water samples
Hyatt C. Green,Richard A. Haugland,Manju Varma,Hana T. Millen,Mark A. Borchardt,Katharine G. Field,William A. Walters,Rob Knight,Rob Knight,Mano Sivaganesan,Catherine A. Kelty,Orin C. Shanks +11 more
TL;DR: The new TaqMan HF183/BacR287 assay should provide more accurate estimations of human-derived fecal contaminants in ambient surface waters.
Journal ArticleDOI
Quantitative PCR for Detection and Enumeration of Genetic Markers of Bovine Fecal Pollution
Orin C. Shanks,Emina Atikovic,A. Denene Blackwood,Jingrang Lu,Rachel T. Noble,Jorge W. Santo Domingo,Shawn Seifring,Mano Sivaganesan,Richard A. Haugland +8 more
TL;DR: Experimental evidence is provided that Acceptable assay performance combined with the prevalence of DNA targets across different cattle populations provides experimental evidence that these quantitative assays will be useful in monitoring bovine fecal pollution in ambient waters.
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Evaluation of genetic markers from the 16S rRNA gene V2 region for use in quantitative detection of selected Bacteroidales species and human fecal waste by qPCR.
Richard A. Haugland,Manju Varma,Mano Sivaganesan,Catherine A. Kelty,Lindsay Peed,Orin C. Shanks +5 more
TL;DR: Analysis for multiple individual Bacteroidales species may be useful in identifying human fecal pollution in environmental waters, as suggested by results of this study.