Márcia Eliana da Silva Ferreira

TL;DR: To increase the frequency of homologous recombination, the KU80 homologue in Aspergillus fumigatus was inactivated and deletion had no influence on pathogenicity in a low-dose murine infection model.

TL;DR: Gene family content across Onygenales and related fungi is characterized, and within Paracoccidioides the authors found expansions of the fungal-specific kinase family FunK1, and capabilities for degrading plant and animal substrates suggest a duality in lifestyle that could enable pathogenic species of OnYgenales to transfer from soil to animal hosts.

TL;DR: This work characterized an Aspergillus fumigatus CRZ1 homologue, CrzA and demonstrated its mediation of cellular tolerance to increased concentrations of calcium and manganese, and linked calcineurin activity with asexual developmental induction, finding that CrZA supports appropriate developmental induction in a calcineURin and brlA‐dependent manner in both species.

TL;DR: A decreased mRNA expression of various ergosterol biosynthesis genes; increased mRNA levels of genes involved in a variety of cell functions, such as transporters, transcription factors, proteins involved in cell metabolism, and hypothetical proteins; and the involvement of the cyclic AMP-protein kinase signaling pathway in the increased expression of several of these genes.

TL;DR: The results suggest that overexpression of drug efflux pumps and/or selection of drug target site mutations are at least partially responsible for itraconazole resistance and could be considered mechanisms for the emergence of clinical resistance to this drug.