M
Margaret M. Billingsley
Researcher at University of Pennsylvania
Publications - 22
Citations - 3148
Margaret M. Billingsley is an academic researcher from University of Pennsylvania. The author has contributed to research in topics: Medicine & Biology. The author has an hindex of 10, co-authored 15 publications receiving 700 citations. Previous affiliations of Margaret M. Billingsley include University of Delaware.
Papers
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Journal ArticleDOI
Orthogonal Design of Experiments for Optimization of Lipid Nanoparticles for mRNA Engineering of CAR T Cells.
Margaret M. Billingsley,Alex G. Hamilton,David Mai,Savan K. Patel,Kelsey L. Swingle,Neil C. Sheppard,Carl H. June,Michael J. Mitchell +7 more
TL;DR: In this article, sequential libraries of ionizable lipid nanoparticle (LNP) formulations with varied excipient compositions were screened in comparison to a standard formulation for improved mRNA delivery to T cells with low cytotoxicity, revealing B10 as the top formulation with a 3-fold increase in mRNA delivery.
Journal ArticleDOI
Delivery technologies for T cell gene editing: Applications in cancer immunotherapy.
TL;DR: An overview of gene editing strategies for T cell therapy gene editing can be found in this paper, where the authors discuss recent investigations and clinical trials that have utilized gene editing to enhance the efficacy of T cells and broaden the application of cancer immunotherapies.
Book ChapterDOI
Quantification of siRNA Duplexes Bound to Gold Nanoparticle Surfaces.
Jilian R. Melamed,Rachel S. Riley,Danielle M. Valcourt,Margaret M. Billingsley,Nicole L. Kreuzberger,Emily S. Day +5 more
TL;DR: Methods to conjugate siRNA duplexes to NPs with gold surfaces are presented and how to quantify the resultant amount of siRNA sense and antisense strands loaded onto the NPs using a fluorescence-based assay is described.
Journal ArticleDOI
A Nanoparticle Platform for Accelerated In Vivo Oral Delivery Screening of Nucleic Acids
Rakan El-Mayta,Rui Zhang,Sarah J. Shepherd,Feng Wang,Margaret M. Billingsley,Vadim Dudkin,Donna Klein,Hoang D. Lu,Michael J. Mitchell +8 more
TL;DR: High‐throughput in vivo screening can accelerate the discovery of LNPs for GI tract nucleic acid delivery upon oral administration, and indicates that the presence of an LNP carrier improved b‐DNA delivery to GI tissues, relative to the delivery of naked b‐ DNA.
Journal ArticleDOI
Amniotic fluid stabilized lipid nanoparticles for in utero intra-amniotic mRNA delivery.
William H. Peranteau,Kelsey L. Swingle,Margaret M. Billingsley,Jingbin Wang,Sourav Bose,Brandon White,Rohan Palanki,Apeksha Dave,Savan K. Patel,Ningqiang Gong,Alex G. Hamilton,Mohamad-Gabriel Alameh,Drew Weissman,William H. Peranteau,Michael J. Mitchell +14 more
TL;DR: In this paper, a library of ionizable lipid nanoparticles (LNPs) was used to evaluate how LNP structure affects ex-natal stability in amniotic fluid, and whether a lead candidate identified from these stability measurements enables intra-amniotic mRNA delivery in utero.