Maria Chiara Mimmi

TL;DR: In this paper, site-directed mutagenesis of a laccase produced by Trametes versicolor was performed to improve its catalytic properties considering a strong interaction of the Asp residue in position 206 with the substrate xylidine, and the mutant enzymes in the yeast Yarrowia lipolytica and assayed the transformation of phenolic and non-phenolic substrates.

TL;DR: The roles of two evolutionary conserved Trp residues in relation to beta(2)m structure, function and folding/misfolding are investigated by means of a combined biophysical and functional approach and it is shown that Trp60 plays a functional role in promoting the association of beta( 2)m in class I major histocompatibility complex.

TL;DR: This work presents the first detailed structural characterization and molecular mechanics model of the interaction between a fibrillogenic protein, β2-microglobulin, and a NP, 5 nm hydrophilic citrate-capped gold nanoparticles, and offers possible strategies for controlling the desired effect of NPs on the conformational changes of the proteins, which have significant roles in the fibrillation process.

TL;DR: A more complex folding energy landscape than previously reported for wild type β2m, involving more than a single intermediate species, and shedding new light into the fibrillogenic pathway is revealed.

TL;DR: An analysis of the Nuclear Magnetic Resonance-detectable part of the metabolome in plasma and urine samples, studying 67 subjects with DS and 29 normal subjects as controls selected among DS siblings showed a clear discrimination between the DS and the control groups.