抗原变异可使得多种致病微生物易于逃避宿主免疫应答。表达在感染红细胞表面的恶性疟原虫红细胞表面蛋白1（PfPMP1）与感染红细胞、内皮细胞、树突状细胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作用。每个单倍体基因组var基因家族编码约60种成员，通过启动转录不同的var基因变异体为抗原变异提供了分子基础。

疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A

基礎講座 電気泳動(Electrophoresis)

Computer and Robot Vision Vol. 1, by R.M. Haralick and Linda G. Shapiro, Addison-Wesley, 1992, ISBN 0-201-10887-1.

Computer and Robot Vision

Micro- and nanoscale robots that can effectively convert diverse energy sources into movement and force represent a
rapidly emerging and fascinating robotics research area. Recent advances in the design, fabrication, and operation of micro/nanorobots have greatly enhanced their power, function, and versatility. The new capabilities of these tiny untethered machines indicate immense potential for a variety of biomedical applications. This article reviews recent progress and future perspectives of micro/nanorobots in biomedicine, with a special focus on their potential advantages and applications for directed drug delivery, precision surgery, medical diagnosis, and detoxification. Future success of this technology, to be realized through close collaboration between robotics, medical, and nanotechnology experts, should have a major impact on disease diagnosis, treatment, and prevention.

/pdf/micro-nanorobots-for-biomedicine-delivery-surgery-sensing-23eglg29gr.pdf

Micro/Nanorobots for Biomedicine: Delivery, Surgery, Sensing, and Detoxification.

Advances in paper-based point-of-care diagnostics.

We present artificially motorized sperm cells—a novel type of hybrid micromotor, where customized microhelices serve as motors for transporting sperm cells with motion deficiencies to help them carry out their natural function. Our results indicate that metal-coated polymer microhelices are suitable for this task due to potent, controllable, and nonharmful 3D motion behavior. We manage to capture, transport, and release single immotile live sperm cells in fluidic channels that allow mimicking physiological conditions. Important steps toward fertilization are addressed by employing proper means of sperm selection and oocyte culturing. Despite the fact that there still remain some challenges on the way to achieve successful fertilization with artificially motorized sperms, we believe that the potential of this novel approach toward assisted reproduction can be already put into perspective with the present work.

Cellular Cargo Delivery: Toward Assisted Fertilization by Sperm-Carrying Micromotors

A sperm-driven micromotor is presented as a targeted drug delivery system, which is appealing to potentially treat diseases in the female reproductive tract. This system is demonstrated to be an efficient drug delivery vehicle by first loading a motile sperm cell with an anticancer drug (doxorubicin hydrochloride), guiding it magnetically, to an in vitro cultured tumor spheroid, and finally freeing the sperm cell to deliver the drug locally. The sperm release mechanism is designed to liberate the sperm when the biohybrid micromotor hits the tumor walls, allowing it to swim into the tumor and deliver the drug through the sperm–cancer cell membrane fusion. In our experiments, the sperm cells exhibited a high drug encapsulation capability and drug carrying stability, conveniently minimizing  toxic side effects and unwanted drug accumulation in healthy tissues. Overall, sperm cells are excellent candidates to operate in physiological environments, as they neither express pathogenic proteins nor proliferate to...

Sperm-Hybrid Micromotor for Targeted Drug Delivery

Mariana Medina-Sanchez and Oliver G. Schmidt set priorities for more realistic tests of tiny machines that could be used to diagnose and treat conditions.

Medical microbots need better imaging and control

An innovative concept for the fabrication of dual-action microrobots capable of performing single-cell microsurgery along with a site-directed drug-delivery feature is presented. These multi-action plant-derived biocompatible "medibots" can play a pivotal role in understanding micromotor interactions at the cellular level, aiming toward the destruction of harmful cells (like cancer) among others in living systems.

Medibots: Dual-Action Biogenic Microdaggers for Single-Cell Surgery and Drug Release

Systemic chemotherapy remains the backbone of many cancer treatments. Due to its untargeted nature and the severe side effects it can cause, numerous nanomedicine approaches have been developed to overcome these issues. However, targeted delivery of therapeutics remains challenging. Engineering microrobots is increasingly receiving attention in this regard. Their functionalities, particularly their motility, allow microrobots to penetrate tissues and reach cancers more efficiently. Here, we highlight how different microrobots, ranging from tailor-made motile bacteria and tiny bubble-propelled microengines to hybrid spermbots, can be engineered to integrate sophisticated features optimised for precision-targeting of a wide range of cancers. Towards this, we highlight the importance of integrating clinicians, the public and cancer patients early on in the development of these novel technologies.

/pdf/engineering-microrobots-for-targeted-cancer-therapies-from-a-imodv8s6jv.pdf

Mariana Medina-Sánchez

Papers

Cellular Cargo Delivery: Toward Assisted Fertilization by Sperm-Carrying Micromotors

Sperm-Hybrid Micromotor for Targeted Drug Delivery

Medical microbots need better imaging and control

Medibots: Dual-Action Biogenic Microdaggers for Single-Cell Surgery and Drug Release

Engineering microrobots for targeted cancer therapies from a medical perspective