Showing papers by "Marie-Thérèse Nugeyre published in 1984"
TL;DR: Virus production was associated with impaired proliferation, modulation of T3-T4 cell markers, and the appearance of cytopathic effects, providing evidence for the involvement of LAV in AIDS.
Abstract: Lymphadenopathy associated virus ( LAV ) has been isolated from patients with the acquired immunodeficiency syndrome (AIDS) or lymphadenopathy syndrome. Since the immune deficiency in AIDS seems to be primarily related to the defect of the helper-inducer T lymphocyte subset, the possibility that LAV is selectively tropic for this subset was investigated. Fractionation of T lymphocytes was achieved by cellular affinity chromatography with monoclonal antibodies. In a hemophilic patient who was a healthy carrier of LAV , reverse transcriptase activity and virus particles detected by electron microscopy were found only in cultures of helper-inducer lymphocytes. When infected with LAV in vitro, lymphocyte subsets from normal individuals yielded similar results. Virus production was associated with impaired proliferation, modulation of T3-T4 cell markers, and the appearance of cytopathic effects. The results provide evidence for the involvement of LAV in AIDS.
937 citations
TL;DR: A strain of lymphadenopathy associated retrovirus passaged in vitro was used to infect a lymphoblastoid cell line obtained by transformation with Epstein-Barr virus of B lymphocytes from a healthy donor, and this adapted strain retained the biochemical, ultrastructural, and antigenic characteristics of the original strain.
Abstract: A strain of lymphadenopathy associated retrovirus ( LAV ) passaged in vitro was used to infect a lymphoblastoid cell line obtained by transformation with Epstein-Barr virus of B lymphocytes from a healthy donor. The virus produced from this line (B- LAV ) was also able to grow at a high rate in some other lymphoblastoid lines and in a Burkitt lymphoma line. This adapted strain retained the biochemical, ultrastructural, and antigenic characteristics of the original strain, as well as its tropism for normal T4+ lymphocytes. It is thus possible to grow LAV in large quantities that can be used for the preparation of diagnostic reagents. The interaction between such a human retrovirus and Epstein-Barr virus, a DNA virus, may have some implication for the pathology of the acquired immunodeficiency syndrome and related diseases.
345 citations
TL;DR: Since this virus has been isolated from patients in other AIDS risk categories, the finding of the virus in AIDS patients from the African group adds further support to the hypothesis that this human retrovirus is the AIDS aetiological agent.
100 citations
TL;DR: Further characterization of a human lymphotropic retrovirus previously isolated from a patient with lymphadenopathy syndrome is reported, which has a distinct morphology when examined by electron microscopy, close to that of equine infectious anaemia virus (EIAV).
Abstract: Summary Further characterization of a human lymphotropic retrovirus previously isolated from a patient with lymphadenopathy syndrome is reported. The virus is not antigenically related to human T-cell leukaemia virus (HTLV1). The mature virions have a distinct morphology when examined by electron microscopy, with a small eccentric core. This morphology is close to that of equine infectious anaemia virus (EIAV). Moreover, the p25 protein of the human isolate can be immunoprecipitated by sera of horses infected with EIAV. Similar viral isolates have been made from several patients with acquired immune deficiency syndrome (AIDS). Antibodies to such viruses are widely distributed in patients having AIDS or at risk of AIDS.
87 citations
Patent•
14 Sep 1984
TL;DR: In this paper, a methode for le diagnostic in vitro du SLA ou du SIDA is proposed, selon lequel on met en contact un serum provenant du malade qui doit etre l'objet du diagnostic avec un retrovirus T-lymphotrope, qui contient une proteine p25 qui n'est pas reconnue immunologiquement par la proteinep24 du virus HTLV and que l'on detecte la reaction immunologique, s'il y en a.
Abstract: La presente invention est relative a une methode pour le diagnostic in vitro du SLA ou du SIDA selon lequel on met en contact un serum provenant du malade qui doit etre l'objet du diagnostic avec un retrovirus T-lymphotrope, qui contient une proteine p25 qui n'est pas reconnue immunologiquement par la proteine p24 du virus HTLV et que l'on detecte la reaction immunologique, s'il y en a. Elle concerne egalement des kits pour le dosage in vivo du SLA ou du SIDA.