M
Mariko Takayama
Researcher at University of Tsukuba
Publications - 16
Citations - 1064
Mariko Takayama is an academic researcher from University of Tsukuba. The author has contributed to research in topics: Glutamate decarboxylase & Cas9. The author has an hindex of 9, co-authored 13 publications receiving 723 citations.
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Journal ArticleDOI
Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion
Zenpei Shimatani,Sachiko Kashojiya,Mariko Takayama,Rie Terada,Takayuki Arazoe,Hisaki Ishii,Hiroshi Teramura,Tsuyoshi Yamamoto,Hiroki Komatsu,Kenji Miura,Hiroshi Ezura,Keiji Nishida,Tohru Ariizumi,Akihiko Kondo +13 more
TL;DR: A fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants demonstrates the feasibility of base editing for crop improvement.
Journal ArticleDOI
Efficient increase of ɣ-aminobutyric acid (GABA) content in tomato fruits by targeted mutagenesis
TL;DR: This is the first study describing the application of the CRISPR/Cas9 system to increase GABA content in tomato fruits, and its findings provide a basis for the improvement of other types of crop by CRISpr/ Cas9-based genetic modification.
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How and why does tomato accumulate a large amount of GABA in the fruit
Mariko Takayama,Hiroshi Ezura +1 more
TL;DR: The molecular mechanism of GABA accumulation and the physiological function of GABA during tomato fruit development remain unclear, and the potential biological roles of GABA in Tomato fruit development are discussed.
Journal ArticleDOI
Suppression of γ-aminobutyric acid (GABA) transaminases induces prominent GABA accumulation, dwarfism and infertility in the tomato (Solanum lycopersicum L.).
TL;DR: Results indicate that pyruvate- and glyoxylate-dependent GABA-T is the essential isoform for GABA metabolism in tomato plants and that GABA- T1 primarily contributes to GABA reduction in the ripening fruits.
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Accumulation Mechanism of γ-Aminobutyric Acid in Tomatoes (Solanum lycopersicum L.) under Low O2 with and without CO2
Nobukazu Mae,Yoshio Makino,Seiichi Oshita,Yoshinori Kawagoe,Atsushi Tanaka,Koh Aoki,Atsushi Kurabayashi,Takashi Akihiro,Kazuhito Akama,Satoshi Koike,Mariko Takayama,Chiaki Matsukura,Hiroshi Ezura +12 more
TL;DR: It is demonstrated that upregulation of GAD activity and downregulation of GABA-TK activity cause GABA accumulation in tomatoes stored under low-O(2) conditions and the effect of CO( 2) on GABA accumulation is probably minimal.