Hiroshi Ezura

TL;DR: A fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants demonstrates the feasibility of base editing for crop improvement.

TL;DR: A highly efficient protocol for the Agrobacterium-mediated genetic transformation of a miniature dwarf tomato (Lycopersicon esculentum), Micro-Tom, a model cultivar for tomato functional genomics, which could become a powerful tool forfunctional genomics in tomato.

TL;DR: The collection of Micro-Tom nrFLcDNAs generated in this study will serve as a valuable genomic tool for plant biologists to bridge the gap between basic and applied studies and aid in tomato functional genomics and molecular breeding.

TL;DR: The CRISPR/Cas9 system is optimized to introduce somatic mutations effectively into SlIAA9—a key gene controlling parthenocarpy—with mutation rates of up to 100% in the T0 generation, and analysis of off-target mutations using deep sequencing indicated that the customized gRNAs induced no additional mutations in the host genome.

TL;DR: Genetic analysis of backcrosses indicated the successful inheritance of the mutations in BC1F2 populations, confirming the reproducibility in the morphological phenotyping of the M2 plants and developed the in silico database TOMATOMA, a relational system interfacing modules between mutant line names and phenotypic categories.