Marina E. Komarovskaya

TL;DR: An efficient vectorfree method of mRNA production from polymerase chain reaction-generated DNA templates is reported that can be applied to generate autologous T lymphocytes directed toward malignant cells and conferred powerful cytotoxicity to T cells against CD19+ targets from the same donor.

TL;DR: It is demonstrated that a mixed population of cytotoxic lymphocytes, including T cells together with NK cells, can be quickly and simultaneously reprogrammed by mRNA against autologous malignancies.

TL;DR: In this article, a method of mRNA production for use in transfection is provided, that involves in vitro transcription of PCR generated templates with specially designed primers, followed by polyA addition, to produce a construct containing 3' and 5' untranslated sequence ('UTR'), a 5' cap and/or Internal Ribosome Entry Site (IRES), the gene to be expressed, and a polyA tail, typically 50-2000 bases in length.

TL;DR: In this paper, compositions and methods of making cells using RNA, and cells made using the disclosed compositions and method are also provided, in exemplary embodiments, RNA is transfected into cells to effect a molecular, biological, physiological, or histological change in the cells.

TL;DR: In vivo, anti-CD19-CIR transfected T cells were able to treat established tumors as demonstrated by non-invasive serial imaging of luciferase-mediated bioluminescence and provide a rationale for developing methods for large-scale electroporation of chimeric receptors in CTLs in support of clinical application of this treatment modality in patients with high-risk B-cell malignancies.