Markus Häner

TL;DR: The functional role of the non-helical end domains of vimentin on its assembly properties using truncated Xenopus and human recombinant proteins suggests that assembly occurred by a principally similar mechanism involving the end-on-fusion or annealing of unit-length filaments.

TL;DR: The mass-per-length (MPL) composition of distinct early assembly products of recombinant intermediate filament (IF) proteins from the four cytoplasmic sequence homology classes is determined, indicating that individual types of IF proteins generate filaments with distinctly different numbers of molecules per cross-section.

TL;DR: Phosphorylation of chicken lamin B2 by cdc2 kinase interferes with the head-to-tail polymerization of the lamin dimers, which supports the notion that cdc1 kinase plays a major, direct role in triggering mitotic disassembly of the nuclear lamina.

TL;DR: It is proposed that hCT aggregation begins with the formation of approximately 4-nm-thick protofibrils, which interact via lateral association and coiling to form higher-order fibrillar assemblies, i.e., prot ofibril-ribbons, fibrils and tubes, tubes, and multistranded cables.

TL;DR: It is indicated that the YRKLLEGEE-motif is crucial for the formation of authentic tetrameric complexes and also for the control of filament width, rather than elongation, during assembly.