Marta Mancilla

TL;DR: Antibody cross-reactivities in the present work suggest that conserved epitopes from S. mansoni ATP diphosphohydrolase are present in this family of nucleotide-splitting enzymes.

TL;DR: Two homogeneous isoenzymes of apyrase from Pimpernel and Desiree varieties of Solanum tuberosum were obtained by affinity chromatography on agarose-Cibacron Blue or agaroses-ATP-phosphonate columns.

TL;DR: Apyrase bound to microsomes from rat and potato tissues was characterized in its substrate specificity and effect of inhibitors, and a characteristic common to the microsomal and soluble apyrases is the stimulatory effect of a potato activator protein of soluble plant apyrase.

TL;DR: Kinetic and physico-chemical studies on human placental microsomal fraction confirmed that the ATPase and ADPase activities detected in this fraction correspond to the enzyme ATP-diphosphohydrolase or apyrase, which is supported by the direct effect on washed platelets and by its plasma membrane localization.

TL;DR: Calcium-stimulated ATPase-ADPase activities were studied in a microsomal fraction of rat placental tissue and the substrate analogue, 5'-(beta, gamma-methylene)triphosphate, protected both enzyme activities against all the modifying amino acid reagents tested.