M
Martin Badertscher
Researcher at ETH Zurich
Publications - 13
Citations - 2503
Martin Badertscher is an academic researcher from ETH Zurich. The author has contributed to research in topics: Mass spectrometry & Proton NMR. The author has an hindex of 9, co-authored 13 publications receiving 2381 citations.
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Book
Structure Determination of Organic Compounds
TL;DR: In this paper, the authors present a combination table of C NMR Spectroscopy, H NMR and Heteronuclear NMR spectroscopy with IR and Mass Spectrometry.
Journal ArticleDOI
Binding constant determination of high-affinity protein-ligand complexes by electrospray ionization mass spectrometry and ligand competition.
TL;DR: For the first time, dissociation constants lower than picomolar could be determined with good accuracy by electrospray ionization mass spectrometry, and the presented methodology relies only on the determination of signal intensity ratios for free ligands in the low mass region.
Book ChapterDOI
1H NMR Spectroscopy
TL;DR: In this article, a double or triple bond next to the CH2 group causes an increase in the number of |J|-emstones. But the effect of substitutions was not as strong.
Journal ArticleDOI
Critical evaluation of mass spectrometric measurement of dissociation constants: accuracy and cross-validation against surface plasmon resonance and circular dichroism for the calmodulin–melittin system
TL;DR: A comparison of MS methods against established methods such as surface plasmon resonance (SPR) and circular dichroism (CD) whose suitability for the quantitative assessment of noncovalent interactions is well known is reported.
Journal ArticleDOI
Determination of protein-ligand binding constants of a cooperatively regulated tetrameric enzyme using electrospray mass spectrometry.
Dragana Cubrilovic,Wolfgang Haap,Konstantin Barylyuk,Armin Ruf,Martin Badertscher,Marcel Gubler,Tim Tetaz,Catherine Joseph,Jörg Benz,Renato Zenobi +9 more
TL;DR: The benefits of nano electrospray ionization mass spectrometry (nanoESI-MS) as a fast and label-free method not only for determination of dissociation constants (KD) of a cooperatively regulated enzyme but also to better understand the mechanism of enzymatic cooperativity of multimeric proteins.