Martina Inga Kirsch

TL;DR: It is demonstrated that the introduction of a polypeptide linker between the fragment difficult (Fd) and the light chain (LC), resulting in the formation of a single chain Fab fragment (scFab), can lead to improved production of functional molecules.

TL;DR: This work presents the first comprehensive comparison of V gene subfamily use for all steps of an antibody phage display pipeline, and describes a compatible cassette vector set, allowing in vivo biotinylation, enzyme fusion and Fc fusion.

TL;DR: Crf2 and the corresponding recombinant antibodies offer a novel approach for the early diagnostics of IA caused by A. fumigatus.

TL;DR: The selection of antibodies against a human pathogenic virus from a human naïve scFv antibody gene library using complete, active virus particles as antigen will improve the fast identification of VEEV in case of a biological warfare or terroristic attack or a natural outbreak.

TL;DR: The significant differences found for phage yield, display of Fabs on the phage and expression of soluble Fabs suggest to use a bicistronic vector with an fd-fragment-pIII fusion for the construction of future Fab phage display libraries.