T
Thomas Schirrmann
Researcher at Braunschweig University of Technology
Publications - 75
Citations - 3562
Thomas Schirrmann is an academic researcher from Braunschweig University of Technology. The author has contributed to research in topics: Phage display & Antibody. The author has an hindex of 30, co-authored 75 publications receiving 2842 citations.
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Journal ArticleDOI
Designing Human Antibodies by Phage Display
André Frenzel,Jonas Kügler,Saskia Helmsing,Doris Meier,Thomas Schirrmann,Michael Hust,Stefan Dübel +6 more
TL;DR: Some opportunities and achievements are summarized, e.g., the generation of antibodies which could not be generated otherwise, and the design of antibody properties by different panning strategies, including the adjustment of kinetic parameters.
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Expression of recombinant Antibodies
TL;DR: This review focuses on current antibody production systems including their usability for different applications, and recent developments of glycosylation-engineered yeast, insect cell lines, and transgenic plants are promising to obtain antibodies with “human-like” post-translational modifications.
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Phage display-derived human antibodies in clinical development and therapy
TL;DR: A comprehensive overview about phage display-derived antibodies that are approved for therapy or in clinical development is provided and a selection of these antibodies is described in more detail to demonstrate different aspects of thephage display technology and its development over the last 25 years.
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High level transient production of recombinant antibodies and antibody fusion proteins in HEK293 cells
Volker Jäger,Konrad Büssow,Andreas Wagner,Susanne Weber,Michael Hust,André Frenzel,Thomas Schirrmann +6 more
TL;DR: Transient production of recombinant scFv-Fc antibodies in HEK293-6E in combination with optimized vectors and fed batch shake flasks cultivation is efficient and robust, and integrates well into a high-throughput recombinant antibody generation pipeline.
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A human scFv antibody generation pipeline for proteome research
Michael Hust,Torsten Meyer,Bernd Voedisch,Torsten Rülker,Holger Thie,Aymen El-Ghezal,Martina Inga Kirsch,Mark Schütte,Saskia Helmsing,Doris Meier,Thomas Schirrmann,Stefan Dübel +11 more
TL;DR: This work presents the first comprehensive comparison of V gene subfamily use for all steps of an antibody phage display pipeline, and describes a compatible cassette vector set, allowing in vivo biotinylation, enzyme fusion and Fc fusion.