M
Matthew B. Begemann
Researcher at University of Wisconsin-Madison
Publications - 26
Citations - 1211
Matthew B. Begemann is an academic researcher from University of Wisconsin-Madison. The author has contributed to research in topics: CRISPR & Gene. The author has an hindex of 12, co-authored 24 publications receiving 1001 citations. Previous affiliations of Matthew B. Begemann include Wisconsin Alumni Research Foundation & University of Missouri.
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Journal ArticleDOI
Synthetic Biology Toolbox for Controlling Gene Expression in the Cyanobacterium Synechococcus sp. strain PCC 7002
TL;DR: The presented synthetic biology toolbox will enable accelerated engineering of PCC 7002 and have a 48-fold dynamic range and was shown to out-perform Ptrc constructs.
Journal ArticleDOI
Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System
Ryan Marshall,Colin S. Maxwell,Scott P. Collins,Thomas Jacobsen,Michelle L. Luo,Matthew B. Begemann,Benjamin Neil Gray,Emma January,Anna Singer,Yonghua He,Chase L. Beisel,Vincent Noireaux +11 more
TL;DR: The use of E. coli cell-free transcription-translation (TXTL) systems are presented to vastly improve the speed and scalability of CRISPR characterization and validation.
Journal ArticleDOI
CRISPR interference as a titratable, trans-acting regulatory tool for metabolic engineering in the cyanobacterium Synechococcus sp. strain PCC 7002
Gina C. Gordon,Travis C. Korosh,Jeffrey C. Cameron,Andrew L. Markley,Matthew B. Begemann,Brian F. Pfleger +5 more
TL;DR: This work is the first example of titratable repression in cyanobacteria using CRISPRi, enabling dynamic regulation of essential processes and manipulation of flux through central carbon metabolism and carbon flux in the production of high-value chemicals.
Journal ArticleDOI
Modular synthase-encoding gene involved in α-olefin biosynthesis in Synechococcus sp. strain PCC 7002.
TL;DR: A gene involved in the production of medium-chain α-olefins was identified in the cyanobacterium Synechococcus sp.
Journal ArticleDOI
Precise insertion and guided editing of higher plant genomes using Cpf1 CRISPR nucleases
Matthew B. Begemann,Benjamin Neil Gray,Emma January,Gina C. Gordon,Yonghua He,Haijun Liu,Xingrong Wu,Thomas P. Brutnell,Todd C. Mockler,Mohammed Oufattole +9 more
TL;DR: The Cpf1 nucleases from Francisella novicida and Lachnospiraceae bacterium ND2006 were screened for their capability to induce targeted gene insertion via homology directed repair and were demonstrated to generate precise gene insertions as well as indel mutations at the target site in the rice genome.