Melissa Alvarez

TL;DR: Two HPLC methods are used with evaporative light scattering detection (ELSD) and mass spectrometry (MS) to characterize polysorbate from commercial vendors to determine the rate of hydrolysis for each polyoxyethylene sorbitan ester of polys orbite 20 in basic solution at room temperature.

TL;DR: The use of peptide map analysis by reversed-phase high-performance liquid chromatography and high-resolution mass spectrometry to detect and identify mistranslation events in recombinant monoclonal antibodies expressed in mammalian cell lines including Chinese hamster ovary (CHO) cells shows a new ability to discover and characterize low level protein variants that arise from mistranslated events.

TL;DR: Here it is demonstrated the utility of a generic on-line liquid chromatography-mass spectrometry (LC-MS) method operated in a two-dimensional format toward the rapid characterization of MAb charge and size variants, enabling high-throughput analysis with minimal sample handling.

TL;DR: The first application of this high resolution technique for characterizing the structure of a therapeutic monoclonal antibody (mAb) dimer is described, showing that peptides displaying decreased rates of oxidation in the dimer are localized in the light and heavy chains of the Fab domain.

TL;DR: In this paper , a new peak detection (NPD) method is proposed for sensitive and unbiased detection of new or changing site-specific attributes between a sample and reference that is not possible with conventional UV or fluorescence detection-based methods.