Journal ArticleDOI
A Robust Purity Method for Biotherapeutics Using New Peak Detection in an LC-MS-Based Multi-Attribute Method.
Monica Sadek,Benjamin Nathan Moore,Christopher Yu,Nicholas Ruppe,Austin Abdun-Nabi,Zhiqi Hao,Melissa Alvarez,Sanket N. Dahotre,Galahad Deperalta +8 more
TLDR
In this paper , a new peak detection (NPD) method is proposed for sensitive and unbiased detection of new or changing site-specific attributes between a sample and reference that is not possible with conventional UV or fluorescence detection-based methods.About:
This article is published in Journal of the American Society for Mass Spectrometry.The article was published on 2023-02-21. It has received 3 citations till now. The article focuses on the topics: Medicine & False positive paradox.read more
Citations
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Journal ArticleDOI
Technical considerations for the implementation of the Multi-Attribute-Method by mass spectrometry in a Quality Control laboratory.
T. Pohl,Annick Gervais,Eef H. C. Dirksen,Karoline Bechtold-Peters,William Burkitt,Li Cao,Simone Greven,Andrew Lennard,Xia Li,Christopher Lößner,Dietmar Reusch,Tomas O’Riordan,Wei Xu,Linda Yi +13 more
TL;DR: In this paper , current literature related to the development and application of the multi-attribute method by peptide mapping liquid chromatography mass spectrometry (MAM) is compiled with the aim of providing guidance for the implementation of MAM in a QC laboratory.
Journal ArticleDOI
A Highly Efficient Workflow for Detecting and Identifying Sequence Variants in Therapeutic Proteins with a High Resolution LC-MS/MS Method
TL;DR: In this paper , a highly efficient sequence variant analysis (SVA) workflow using high-resolution MS and tandem mass spectrometry combined with improved software to greatly reduce the time and resource cost associated with MS SVA workflows was presented.
Journal ArticleDOI
Mass spectrometry-based multi-attribute method in protein therapeutics product quality monitoring and quality control.
Feng Yang,Jennifer Hongyang Zhang,Monica Sadek,Zhiqi Hao,Dietmar Reusch,Wayman Chan,Anja Bathke,Hilary Pallat,Victor F. Lundin,J. Felix Kepert,Patrick Bulau,Galahad Deperalta,Christopher Yu,Tura C. Camilli,Reed J. Harris,John T. Stults +15 more
TL;DR: The multi-attribute method (MAM), a liquid chromatography-mass spectrometry (LC-MS)-based peptide mapping method, has gained increased interest and applications in the biopharmaceutical industry as discussed by the authors .
References
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Journal ArticleDOI
Development of therapeutic antibodies for the treatment of diseases
TL;DR: The preeminent antibody engineering technologies used in the development of therapeutic antibody drugs, such as humanization of monoclonal antibodies, phage display, the human antibody mouse, single B cell antibody technology, and affinity maturation are outlined.
Journal ArticleDOI
Electrospray Ionization Mass Spectrometry: A Technique to Access the Information beyond the Molecular Weight of the Analyte
TL;DR: The present review has described the development of Electrospray Ionization mass spectrometry (ESI-MS) during the last 25 years in the study of various properties of different types of biological molecules.
Journal ArticleDOI
Orbitrap Mass Spectrometry
TL;DR: Orbitrap is the newest addition to the family of high-resolution mass spectrometry analyzers, combining high speed with excellent quantification properties, ranking favorably in many analytical applications.
Journal ArticleDOI
Identification and characterization of asparagine deamidation in the light chain CDR1 of a humanized IgG1 antibody.
Josef Vlasak,Marie Claire Bussat,Shiyi Wang,Elsa Wagner-Rousset,Mark Schaefer,Christine Klinguer-Hamour,Marc Kirchmeier,Nathalie Corvaia,Roxana Ionescu,Alain Beck +9 more
TL;DR: Asparagine deamidation in light chain complementarity determining region 1 of a humanized IgG1 monoclonal antibody is identified and both Asp and isoAsp are assigned, which were assigned by Edman degradation and byisoAsp detection using protein isoaspartate methyltransferase.
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