A simple highly selective ratiometric fluorescent probe for detection of peroxynitrite and its bioimaging applications

Rational design of a water-soluble TICT-AIEE-active fluorescent probe for mercury ion detection.

Recent advances in small-molecule fluorescent probes for diagnosis of cancer cells/tissues

Nickel, as a transition metal, is widely used in industrial production and distributed in human body as an essential micronutrient. But the excessive presence of nickel can also cause pollution to the environment and produce harm to human body. Hence, the sensitive detection of metal nickel has attracted widespread attention. Compared with other heavy metal ions, such as mercury ions, lead ions, chromium ions, the reported fluorescence probes for nickel ions are relatively less and lack of simple and effective detection methods. In addition, most of the reported fluorescent probes for nickel ions are of coordination type, which leads to some limitations in sensitivity and selectivity. Therefore, we constructed a reactive fluorescent probe FA-Ni to achieve highly selective and ultra-sensitive recognition of nickel ions. The probe FA-Ni can quickly and sensitively recognize nickel ions (DL = 1.87 nM), avoid the interference of other ions, so as to realize the selective detection of nickel ions. Meanwhile, probe FA-Ni can be used for the detection of nickel ions in environmental water samples and soil samples (recovery rates > 90%), indicating that FA-Ni is of great significance in environmental applications. The probe FA-Ni can also be applied to the detection of nickel ions in living cells and achieve in-situ imaging, which is expected to provide an important auxiliary tool for the diagnosis of related diseases and a powerful means to understand the physiological function of nickel ions. • Probe FA-Ni could quantitatively and sensitively detect Ni 2+ with low detection limit (DL = 1.87 nM). • The color change of the solution after reaction can be observed with the naked eye. • Probe FA-Ni has excellent selectivity for Ni 2+ compared to other interfering ions. • Probe FA-Ni was successfully applied to detect Ni 2+ in environmental water samples, soil samples and living cells. 

An ultra-sensitive and highly selective fluorescent probe for nickel ions and its environmental and biological applications

A novel ratiometric fluorescent probe for the detection of nickel ions in the environment and living organisms.

https://doi.org/10.1016/j.scitotenv.2022.156164

A new-type HOCl-activatable fluorescent probe and its applications in water environment and biosystems

Cancer, as a malignant tumor, seriously endangers human health. The study of cancer diagnosis and therapy has great practical significance. The development of theranostic agents has become a very important research topic. Nevertheless, some existing agents still have imperfections, such as complex structures and difficult syntheses. Therefore, it is urgent for researchers to develop simple novel theranostic agents. In this study, the precipitated fluorophore HAPQ was used as a simple drug molecule for the first time and combined with NBD-Cl to construct a simple and efficient theranostic probe (HAPQ-NBD). The theranostic probe can distinguish between tumor cells and normal cells based on the higher levels of biothiol in tumor cells. In addition, the probe can use biothiol as a control switch to release higher levels of precipitated fluorophore HAPQ in tumor cells, leading to selective high toxicity to tumor cells, thus achieving the goal of selectively killing tumor cells. The construction of probe HAPQ-NBD provides a practical tool for the diagnosis and therapy of cancer. It is expected that the development and utilization of precipitated fluorophore will provide a new method and strategy for cancer diagnosis and therapy.

Miaohui Yu

Papers

A new-type HOCl-activatable fluorescent probe and its applications in water environment and biosystems

Concise Biothiol-Activatable HPQ-NBD Conjugate as a Targeted Theranostic Probe for Tumor Cells.

An ultra-sensitive and highly selective fluorescent probe for nickel ions and its environmental and biological applications

Portable ratiometric fluorescence analytical device for copper ions based on smartphone in environment and living organisms.

A novel ratiometric fluorescent probe for the detection of nickel ions in the environment and living organisms.