Michael E. Dailey

TL;DR: It is shown that microglia from mice lacking Gi-coupled P2Y12 receptors exhibit normal baseline motility but are unable to polarize, migrate or extend processes toward nucleotides in vitro or in vivo, implying that P2 Y12 is a primary site at which nucleotide act to induce microglial chemotaxis at early stages of the response to local CNS injury.

TL;DR: Time-lapse fluorescence confocal microscopy was used to directly visualize the formation and dynamics of postsynaptic target structures (i.e., dendritic branches and spines) on pyramidal neurons within developing hippocampal tissue slices, revealing a highly dynamic state of post synapse target structures that may actively contribute to the formation of synaptic connections during CNS development.

TL;DR: The rapid conversion of resting ramified microglia to active amoeboid macrophages is accomplished not by converting quiescent branches to dynamic ones, but rather by replacing existing branches with an entirely new set of highly motile protrusions.

TL;DR: Time-lapse confocal microscopy was used to observe directly the dynamic behaviors of migrating cells in living slices of developing cortex, and the majority of cells migrated along a radial pathway, consistent with the view that cortical neurons migrate along radial glial fibers.

TL;DR: Time lapse imaging of transfected neurons expressing GFP-tagged PSD95, a prominent PSD protein, revealed that up to 40% of PSDs in developing dendrites are structurally dynamic; they rapidly appear or disappear, but also grow, shrink and move within shafts and spines.