Showing papers by "Michael Egholm published in 1992"

Peptide nucleic acids

Abstract: A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker.

The use of nucleic acid analogues in diagnostics and analytical procedures

Abstract: Methods of capture, recognition, detection, identification or quantitation of nucleic acids and diagnostics uses generally are described in which are used: (a) a peptide nucleic acid (PNA) comprising a polyamide backbone bearing a plurality of ligands at respective spaced locations along said backbone, said ligands being each independently naturally occurring nucleobases, non-naturally occurring nucleobases or nucleobase-binding groups, each said ligand being bound directly or indirectly to a nitrogen atom in said backbone, and said ligand bearing nitrogen atoms mainly being separated from one another in said backbone by from 4 to 8 intervening atoms; (b) a nucleic acid analogue capable of hybridising to a nucleic acid of complementary sequence to form a hybrid which is more stable against denaturation by heat than a hybrid between the conventional deoxyribonucleotide corresponding to said analogue and said nucleic acid; or (c) a nucleic acid analogue capable of hybridising to a double stranded nucleic acid in which one strand has a sequence complementary to said analogue, so as to displace the other strand from said one strand Preferred compounds have formula (III), wherein each L is independently selected from the group consisting of hydrogen, phenyl, naturally occurring nucleobases, and non-naturally occurring nucleobases; each R7' is independently selected from the group consisting of hydrogen and the side chains of naturally occurring alpha amino acids; n is an integer from 1 to 60, each k and m is, independently, zero or one; and each 1 is independently from zero to 5; Rh is OH, NH?2? or -NHLysNH2; and R?i? is H or COCH?3?

Selective Hydrogenolysis of Bis(hydroxymethyl)aromatic Compounds

Abstract: Selective hydrogenolysis of symmetric bis(hydroxymethyl)aromatic compounds has been performed over Raney nickel in alkaline solution for 24-36 h at 20 o C. The yield of the corresponding monohydrogenolysis product was in the range of 75-96%. The usefulness of the reaction was demonstrated by the synthesis of 4-(bromomethyl)benzoic acid