Michael J. Allison

TL;DR: In this article, the USGS's Ecosystems Mission Area, Invasive Species Program, intramural research program of the U.S. Department of Agriculture, Animal and Plant Health Inspection Service, Wildlife Services, National Wildlife Research Center and an Ignite Grant #IGN07171717 from Innovate BC.

TL;DR: In this article, the authors evaluate six steps in the qPCR-based eDNA assay development and validation workflow and identify the need for more fulsome mitochondrial genome sequence information for a broader range of species; and bring solutions toward best practices in forthcoming large-scale and worldwide eDNA applications, such as at risk or invasive species assessments and site remediation monitoring.

TL;DR: The present study rigorously validated a quantitative real-time polymerase chain reaction (qPCR)-based tool for detecting coastal tailed frog eDNA in water samples and confirms a previously suspected, apparently isolated coastal tails frog metapopulation in the Shulaps drainage.

TL;DR: This work describes the design and validation of a new quantitative real-time polymerase chain reaction (qPCR)-based eDNA eCOTE3 assay with high specificity and sensitivity for sharp-tailed snake, an endangered species under the Canadian Species at Risk Act.