Michael J. Garabedian

TL;DR: This work has taken advantage of the capacity of mammalian steroid receptors to function in yeast and constructed a strain of Saccharomyces cerevisiae in which hsp90 expression was regulatable and could be reduced more than 20-fold relative to wild type, providing the first biological evidence that hSp90 acts in the signal transduction pathway for steroid receptors.

TL;DR: In this article, the exact boundaries of the steroid binding domain of glucocorticoid receptors have been examined with a variety of receptor deletion constructs, and it was shown that three independent regions appear to be required for the generation of the steroids binding form of receptors.

TL;DR: V vectors for the expression of mammalian receptors in yeast, reporter genes, yeast host strains, and simple assays that monitor receptor transcriptional activity are described.

TL;DR: It is shown here that GRs from v-mos-transformed cells are hyperphosphorylated on a specific peptide and maintain hormone-induced phosphorylations upon a prolonged hormone treatment that is associated with disruptions in its nucleocytoplasmic shuttling.

TL;DR: In this paper, the equivalent of the cysteine-656 of the rat glucocorticoid receptor was altered to either serine or glycine for the production of super-glucocyte receptors which retain full biological activity in intact cells and have higher affinity and specificity for glucoc corticoid steroid binding than the original receptor.