M
Michael J. Shamblott
Researcher at Johns Hopkins University School of Medicine
Publications - 35
Citations - 4557
Michael J. Shamblott is an academic researcher from Johns Hopkins University School of Medicine. The author has contributed to research in topics: Stem cell & Embryoid body. The author has an hindex of 19, co-authored 34 publications receiving 4492 citations.
Papers
More filters
Journal ArticleDOI
Derivation of pluripotent stem cells from cultured human primordial germ cells
Michael J. Shamblott,Joyce Axelman,Shunping Wang,Elizabeth M. Bugg,John W. Littlefield,Peter J. Donovan,Paul D. Blumenthal,George R. Huggins,John D. Gearhart +8 more
TL;DR: In this article, a set of primordial germ cells (PGCs, 5-9 weeks postfertilization) were cultured on mouse STO fibroblast feeder layers in the presence of human recombinant leukemia inhibitory factor (HILI) and forskolin.
Derivation of pluripotent stem cells from cultured human primordial germ cells (alkaline phosphataseyembryoid bodyyembryonic stem cellyembryonic germ cell)
Michael J. Shamblott,Joyce Axelman,Shunping Wang,Elizabeth M. Bugg,John W. Littlefield,Peter J. Donovan,Aul D. Blumenthal,R. Huggins,John D. Gearhart +8 more
TL;DR: Based on their origin and demonstrated properties, these human PGC-derived cultures meet the criteria for pluripotent stem cells and most closely resemble EG cells.
Journal ArticleDOI
Human embryonic germ cell derivatives express a broad range of developmentally distinct markers and proliferate extensively in vitro
Michael J. Shamblott,Joyce Axelman,John W. Littlefield,Paul D. Blumenthal,George R. Huggins,Yan Cui,Linzhao Cheng,John D. Gearhart +7 more
TL;DR: The growth and expression characteristics of EB-derived cells suggest that they are relatively uncommitted precursor or progenitor cells, suited to studies of human cell differentiation and may play a role in future transplantation therapies.
Journal ArticleDOI
Derivation of pluripotent stem cells from cultured human primordial germ cells
Michael J. Shamblott,Joyce Axelman,Shunping Wang,Elizabeth M. Bugg,John W. Littlefield,Peter J. Donovan,Paul D. Blumenthal,George R. Huggins,John D. Gearhart +8 more
TL;DR: Based on their origin and demonstrated properties, these human PGC-derived cultures meet the criteria for pluripotent stem cells and most closely resemble EG cells.
Journal ArticleDOI
Human embryonic germ cell derivatives facilitate motor recovery of rats with diffuse motor neuron injury.
Douglas A. Kerr,Jerònia Lladó,Michael J. Shamblott,Nicholas J. Maragakis,David N. Irani,Thomas O. Crawford,Chitra Krishnan,Sonny Dike,John D. Gearhart,Jeffrey D. Rothstein +9 more
TL;DR: It is concluded that cells derived from human pluripotent stem cells have the capacity to restore neurologic function in animals with diffuse motor neuron disease via enhancement of host neuron survival and function.