Michael Oelgeschläger

TL;DR: The use of a Wnt/β-Catenin-reporter ESC line isolated from a previously described mouse line that carries the LacZ reporter gene under the control of a β- Catenin responsive promoter is described.

TL;DR: A standardized protocol for the use of GSEA to distinguish between appropriate and inappropriate animal models for translational research is developed, thus avoiding unnecessary animal experiments and misleading translational studies.

TL;DR: The first years of the 21 st century have seen an unparalleled progress in biomedical sciences, and several new approaches, e.g., stem cell-based, diseases-in- a-dish and organs-on-a-chip and lab-on a-chip technologies have revitalized the domain of alternatives to animal experimentation.

TL;DR: Wittkowski et al. as mentioned in this paper presented a protocol for a 96 h, medium-throughput assay, assessing growth and offspring count quantitatively within an automated framework using open-source software.

TL;DR: This work proposes combining transgenic mouse models harboring easily detectable reporter genes under the control of evolutionarily conserved signaling cascades with existing alternative toxicological testing strategies to enable the plausibility of in vitro data to be verified in light of in vivo data and facilitate regulatory acceptance ofIn vitro test methods.